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In vitro Susceptibility of Streptococcus oralis to Different Mouthwashes

Alessandro Leite Cavalcanti orcid.org/0000-0003-3572-3332 ; Katedra za dječju stomatologiju Stomatološkog fakulteta Državnoga sveučilišta Paraiba, Campina Grande, Brazil
Rafaella Bastos Leite ; Student Stomatološkog fakulteta Državnoga sveučilišta Paraíba, Campina Grande, Brazil
Mariana da Costa Oliveira ; Student Stomatološkog fakulteta Državnoga sveučilišta Paraíba, Campina Grande, Brazil
Alidianne Fábia Cabral Xavier ; Student na Stomatološkog fakulteta Državnoga sveučilišta Paraíba, Campina Grande, Brazil
Ricardo Dias de Castro ; Klinički zavod za javno zdravstvo Stomatološkog sveučilišta João Pessoa u Paraibi, Brazil

Sažetak

Aim: The use of mouthwashes represents, in many cases, the first option for the chemical control of dental biofilm. To evaluate in vitro susceptibility of Streptococcus oralis (ATCC 10557) to commercial mouthwashes available on the Brazilian market. Methods: Seven mouthwashes were evaluated: Equate®, Colgate Plax Overnight®, Sanifill®, Cepacol Cool Citrus® and Oral-B® (cetylpyridinium chloride), Listerine® (Thymol) and Peroxyl® (hydrogen peroxide). A 0.12% chlorhexidine digluconate oral rinse (Periogard®) served as a positive control. The antibacterial activity of the mouthwashes in 7 dilutions (1:0 to 1:32) was evaluated by determining the maximum
inhibitory dilution (MID) in Muller Hinton agar supplemented with 5% blood. Fifty microliters of each ready-to-use solution and their dilutions were poured into wells perforated in the solid culture medium and the dishes were incubated in bacteriological incubators at 37oC for 24 hours. The analysis of MID data was based on the measurement of the zones of bacterial growth inhibition (in mm) formed around the wells (only inhibition zones with a diameter ≥10 mm were considered). Results: The following MIDs were obtained: 1:2 for Equate® and Oral-B®, 1:1 for Sanifill®, and 1:4 for Plax®, Cepacol® and Periogard®. Peroxyl® and Listerine® did not show antibacterial activity against S. oralis. In conclusion, S. oralis presented susceptibility to the mouthwashes evaluated in this study, except for Peroxyl® and Listerine®. Conclusion: These results suggest that cetylpyridinium chloride and chlorhexidine gluconate, which are the most important active components of the tested brands of mouthwashes, are important agents in the chemical control of dental biofilm.

Ključne riječi

Mouthwashes; Streptococcus oralis; Biofilms; Cetylpyridinium; Oral Hygiene; Chlorhexidine Gluconate

Hrčak ID:

94356

URI

https://hrcak.srce.hr/94356

Datum izdavanja:

28.12.2012.

Podaci na drugim jezicima: hrvatski

Posjeta: 3.323 *