Izvorni znanstveni članak

Comparative immunogenicity and protective efficacy of different preparations of outer membrane proteins of Pasteurella multocida (B:2) in a mouse model.

Sumit Joshi ; Department of Molecular Biology and Genetic Engineering, Govind Ballabh Pant University of Agriculture and Technology Pantnagar, Uttarakhand, India
Kamal Tewari ; Department of Veterinary Microbiology, Govind Ballabh Pant University of Agriculture and Technology Pantnagar, Uttarakhand, India
Rashmi Singh ; Department of Veterinary Microbiology, Govind Ballabh Pant University of Agriculture and Technology Pantnagar, Uttarakhand, India

Sažetak

The present study was undertaken to evaluate the protective efficacy of recombinant outer membrane protein (OmpH) of Pasteurella multocida (B:2). The 34 kDa native OmpH protein was purified from OMP-rich extract. Cloning, expression and purification of recombinant OmpH were carried out. Protection studies in mice with sonicated whole cell lysate (Group I), OMP (Group II), native 34 kDa OmpH (Group III) and recombinant OmpH (Group IV), along with a positive (Group V; commercial vaccine) and negative control (Group VI) showed a survivability percentage of 83.33%, 100%, 50%, 63.67% and 83.33% in Groups I-V, respectively, against 100% mortality in Group VI. Serum IgG titres of 30 pooled sera samples from each of the 5 groups, collected at weekly intervals, was assessed using i-ELISA. In Group I, a significant increase was observed in titres on d7, d14 and d21, having mean values (log10 titre) of 2.58, 3.93 and 4.49 respectively. In Group II, there was a significant increase in antibody response on d7, d21 and d28, having mean values of 3.0, 4.04 and 4.41, respectively. In Group III, there was a significant increase in titres on d7, d21 and d28, with a mean titre of 3.70, 4.23 and 4.67, respectively. In Group IV there was a significant increase in titres on d7 and d14, having titre values of 3.36 and 3.58, respectively. In the positive control, a significant increase in titres was obtained on d7 and d14 with titres of 4.06 and 4.41, respectively. Taking these observations together, it may be concluded that OMP plays an immuno-protective role and possesses strong potential for the development of a candidate subunit vaccine against HS.

Ključne riječi

Pasteurella multocida; OMP; OmpH; recombinant; vaccine

Hrčak ID:

111895

URI

https://hrcak.srce.hr/111895

Datum izdavanja:

9.12.2013.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.861 *