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Reumatizam, Vol. 58 No. 1, 2011.

Original scientific paper

The connection between HLA microsatellites and HLA-B*27 gene in patients with psoriatic arthritis in Croatian population

Davor Štimac ; Department for Tissue Typing, Clinical Department for Laboratory Diagnostics, Clinical Hospital Centre Zagreb, Zagreb, Croatia
Zorana Grubić ; Department for Tissue Typing, Clinical Department for Laboratory Diagnostics, Clinical Hospital Centre Zagreb, Zagreb, Croatia
Katarina Štingl ; Department for Tissue Typing, Clinical Department for Laboratory Diagnostics, Clinical Hospital Centre Zagreb, Zagreb, Croatia
Porin Perić ; Clinic for Rheumatic Diseases and Rehabilitation, Clinical Hospital Centre Zagreb, Zagreb, Croatia
Božidar Ćurković ; Clinic for Rheumatic Diseases and Rehabilitation, Clinical Hospital Centre Zagreb, Zagreb, Croatia
Renata Žunec ; Department for Tissue Typing, Clinical Department for Laboratory Diagnostics, Clinical Hospital Centre Zagreb, Zagreb, Croatia

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Abstract

The polymorphism at HLA microsatellites (D6S248, D6S2674, D6S2811 and D6S273) among patients with psoriatic arthritis (PsA) (N=22) and healthy control subjects (K; N=94), as well as haplotypic associations between tested loci were analysed in this study. All subjects were previously typed for HLA-A and -B by PCR-SSP method and were HLA-B*27 positive. HLA microsatellites were analysed using PCR-STR method and electrophoresis in an ALFexpress sequencer. The results demonstrated statistically significant P value for following alleles: D6S273-3 (PsA-21.1 % vs. K-4.9 %; P=0.0013) and D6S273-4 (PsA-15.8 % vs. K-32.1 %; P=0.0180). Analysis of haplotypic associations showed the only statistically significant difference for combination HLA-B*27/D6S273-4 (PsA-10.5 % vs. K-40.2 %; P=0.0164). The results presented in this study lead to the assumption that some other gene/s involved in ethiology of PsA are located in the proximity of D6S273 microsatellite, but in order to reach a final conclusion, an increase in the number of patients is necessary.

Keywords

HLA-B*27 gene; psoriatic arthritis; HLA microsatellites; haplotypic associations

Hrčak ID:

124545

URI

https://hrcak.srce.hr/124545

Publication date:

1.6.2011.

Article data in other languages: croatian

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