Izvorni znanstveni članak
https://doi.org/10.2478/10004-1254-65-2014-2554
Sex-, tissue-, and exposure duration-dependent effects of imidacloprid modulated by piperonyl butoxide and menadione in rats. Part I: oxidative and neurotoxic potentials
Mustafa Yardimci
; Adiyaman University, Institute of Natural and Applied Sciences, Department of Biology, Adiyaman, Turkey
Yusuf Sevgiler
orcid.org/0000-0002-4373-2389
; Adiyaman University, Faculty of Science and Letters, Department of Biology, Adiyaman, Turkey
Eyyup Rencuzogullari
; Adiyaman University, Faculty of Science and Letters, Department of Biology, Adiyaman, Turkey
Mehmet Arslan
; Ardahan University, Health Sciences College, Department of Nursery, Merkez, Ardahan, Turkey
Mehmet Buyukleyla
orcid.org/0000-0002-4450-2710
; Cukurova University, Institute of Natural and Applied Sciences, Department of Biology, Balcali, Adana, Turkey
Mehmet Yilmaz
; Cukurova University, Institute of Natural and Applied Sciences, Department of Biology, Balcali, Adana, Turkey
Sažetak
Earlier research has evidenced the oxidative and neurotoxic potential of imidacloprid, a neonicotinoid insecticide, in different animal species. The primary aim of this study was to determine how metabolic modulators piperonyl butoxide and menadione affect imidacloprid’s adverse action in the liver and kidney of Sprague-Dawley rats of both sexes. The animals were exposed to imidacloprid alone (170 mg kg-1) or in combination with piperonyl butoxide (100 mg kg-1) or menadione (25 mg kg-1) for 12 and 24 h. Their liver and kidney homogenates were analysed spectrophotometrically for glutathione peroxidase, glutathione S-transferase, catalase, total cholinesterase specific activities, total glutathione, total protein content, and lipid peroxidation levels. Imidacloprid displayed its prooxidative and neurotoxic effects predominantly in the kidney of male rats after 24 h of exposure. Our findings suggest that the observed differences in prooxidative and neurotoxic potential of imidacloprid could be related to differences in its metabolism between the sexes. Co-exposure (90-min pre-treatment) with piperonyl butoxide or menadione revealed tissue-specific effect of imidacloprid on total cholinesterase activity. Increased cholinesterase activity in the kidney could be an adaptive response to imidacloprid-induced oxidative stress. In the male rat liver, co-exposure with piperonyl butoxide or menadione exacerbated imidacloprid toxicity. In female rats, imidacloprid+menadione co-exposure caused prooxidative effects, while no such effects were observed with imidacloprid alone or menadione alone. In conclusion, sex-, tissue-, and duration-specific effects of imidacloprid are remarkable points in its toxicity.
Ključne riječi
catalase; cholinesterase; glutathione peroxidase; glutathione S-transferase; neonicotinoids; oxidative stress
Hrčak ID:
130347
URI
Datum izdavanja:
9.12.2014.
Posjeta: 2.819 *