Izvorni znanstveni članak

Cytogenetic Effects of Met-enkephalin (Peptid-M) on Human Lymphocytes

Nikola Štambuk ; Rugjer Bošković Institute, Bijenička 54, HR–10001 Zagreb, Croatia
Nevenka Kopjar ; Institute for Medical Research and Occupational Health, Ksaverska c. 2, HR–10000 Zagreb, Croatia
Karmela Šentija ; Clinical Hospital “Merkur”, Zajčeva 19, HR–10000 Zagreb, Croatia
Vera Garaj-Vrhovac ; Institute for Medical Research and Occupational Health, Ksaverska c. 2, HR–10000 Zagreb, Croatia
Dražen Vikić-Topić ; Rugjer Bošković Institute, Bijenička 54, HR–10001 Zagreb, Croatia
Branka Marušić-Della Marina ; Childrens Hospital Zagreb, Klaićeva 16, HR–10000 Zagreb, Croatia
Vesna Brinar ; Department of Neurology, Clinical Hospital Centre, Zagreb, Kišpatićeva 12, HR–10000 Zagreb, Croatia
Milica Trbojević-Čepe ; Clinical Institute of Laboratory Diagnosis, Clinical Hospital Centre, Zagreb, Kišpatićeva 12, HR–10000 Zagreb, Croatia
Neven Žarković ; Rugjer Bošković Institute, Bijenička 54, HR–10001 Zagreb, Croatia
Božidar Ćurković ; Department of Rheumatology and Rehabilitation, Clinical Hospital Centre, Zagreb, Kišpatićeva 12, HR–10000 Zagreb, Croatia
Ðurđa Babić-Naglić ; Department of Rheumatology and Rehabilitation, Clinical Hospital Centre, Zagreb, Kišpatićeva 12, HR–10000 Zagreb, Croatia
Mirko Hadžija ; Rugjer Bošković Institute, Bijenička 54, HR–10001 Zagreb, Croatia
Niko Zurak ; Department of Neurology, Clinical Hospital Centre, Zagreb, Kišpatićeva 12, HR–10000 Zagreb, Croatia
Zdravko Brzović ; Department of Neurology, Clinical Hospital Centre, Zagreb, Kišpatićeva 12, HR–10000 Zagreb, Croatia
Roko Martinić ; Clinical Hospital Split, Spinčićeva 1, HR–21000 Split, Croatia
Vjera Štambuk ; Clinical Hospital Split, Spinčićeva 1, HR–21000 Split, Croatia
Paško Konjevoda ; Department of Pharmacology, Zagreb University School of Medicine, Šalata 2, HR–10000 Zagreb, Croatia
Nikola Ugrinović ; University Clinic for Diabetes and Metabolic Diseases, Dobri dol 4a, HR–10000 Zagreb, Croatia
Ivana Pavlić-Renar ; University Clinic for Diabetes and Metabolic Diseases, Dobri dol 4a, HR–10000 Zagreb, Croatia
Zdenko Biđin ; Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, HR–10000 Zagreb, Croatia
Biserka Pokrić ; Rugjer Bošković Institute, Bijenička 54, HR–10001 Zagreb, Croatia

Sažetak

The structure, complementary structure and cytogenetic/proliferative effects of the Met-enkephalin on human peripheral blood lymphocytes were analyzed. Met-enkephalin, i.e. Peptid-M (LUPEX®), is a low molecular weight synthetic pentapeptide that corresponds to thymus Met-enkephalin. Its structure was examined by means of NMR spectroscopy. The influence of Met-enkephalin on in vitro normalization of chromosomally aberrant lymphocytes of patients suffering from different immune-mediated diseases, was analyzed by the sensitive cytogenetic tests for screening and detection of genome damages in human lymphocytes. The tests showed that in vitro stimulation of human lymphocytes with the Met-enkephalin led to dissapearance of different types of chromosome aberrations, reduction in the number of micronuclei, decrease in the frequency of sister chromatid exchange (SCE) and apoptosis as well as a cytostatic effect on mitosis cycles. They have also confirmed normalization of chromosomally aberrant cell findings in patients suffering from different immune-mediated diseases. These results suggest a possible role of Met-enkephalin (Peptid-M) in immunotherapy of different diseases which involve chromosomal aberrations as well as abnormal cell proliferation and offer new approaches to immunotherapy by the use of Peptid-M. Based on the molecular recognition theory and the SCA method, peptide complementary to Peptid-M was designed, synthesized and denoted Peptide-D. Peptide-D is a calpastatin fragment. Predicted ligand-receptor interaction between both peptides is confirmed by the results showing that Peptide-D blocked the Peptid-M induced lymphocyte proliferation in a dosedependent manner.

Ključne riječi

Hrčak ID:

132370

URI

https://hrcak.srce.hr/132370

Datum izdavanja:

1.10.1998.

Posjeta: 869 *