Kratko priopćenje

https://doi.org/10.1515/acph-2016-0001

Methods for preliminary determination of pemetrexed in macromolecular drug-carrier systems

JAROSLAW CIEKOT ; “NeoLek” Laboratory, Department of Experimental Oncology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, PAS, 53-114, Wrocław, Poland
TOMASZ MAREK GOSZCZYŃSKI ; “NeoLek” Laboratory, Department of Experimental Oncology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, PAS, 53-114, Wrocław, Poland
JANUSZ BORATYŃSKI ; “NeoLek” Laboratory, Department of Experimental Oncology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, PAS, 53-114, Wrocław, Poland; Jan Dlugosz Academy, Częstochowa 42-201, Poland

Sažetak

Pemetrexed (PMX) is an antifolate drug utilized in the treatment of non-small cell lung cancer. For studies of potential macromolecular carriers for PMX, fast and precise methods were developed to determine the bound and free drug contained in investigated conjugate preparations. The analysis of the total amount of PMX in conjugates was based on absorption spectrophotometry. The linearity was found in the range of 4.697–46.97 µmol L–1 PMX The limit of quantitation was 1.070 µmol L–1. The method for the analysis of unbound PMX was based on size-exclusion chromatography and detection at 225 nm. This method shows linear range of 2.230–223.0 µmol L–1. LOQ was 0.539 µmol L–1. The proposed methods can be used both for the characterization of the polysaccharide based conjugates of PMX and for the determination of conjugate drug release profiles.

Ključne riječi

pemetrexed; macromolecular drug-carrier; size-exclusion chromatography; ultraviolet spectroscopy

Hrčak ID:

145268

URI

https://hrcak.srce.hr/145268

Datum izdavanja:

31.3.2016.

Posjeta: 1.727 *