Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2014.2022
Substrate Inhibition Growth Kinetics for Cutinase Producing Pseudomonas cepacia Using Tomato-peel Extracted Cutin
K. Dutta
; Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology (IIT) Guwahati, Guwahati 781039, Assam, India
V. Venkata Dasu
; Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology (IIT) Guwahati, Guwahati 781039, Assam, India
B. Mahanty
; Department of Environmental Engineering, INHA University, Incheon 402751, Republic of Korea
A. Anand Prabhu
; Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology (IIT) Guwahati, Guwahati 781039, Assam, India
Sažetak
Using tomato-peel extracted cutin, an economically viable substrate, cutinase production by Pseudomonas cepacia was studied at different initial substrate concentrations (2–20 g L–1). The highest volumetric enzyme activity was observed at 10 g L–1 of cutin, which was inhibited at further higher concentrations. Various 3-, 4- and 5- parametric Monod-variant models were chosen to analyze the inhibition kinetics. The model parameters as well as goodness of fit were estimated using non-linear regression analysis. The
4- parameter Webb model was the best-fit model (R2 = 0.933), followed by the 3-parameter Andrews model (R2 = 0.92). Parameter sensitivity analysis revealed that the maximum specific growth rate was the most sensitive parameter for both the models, and the
Webb constant was the least sensitive. Finally, based on a strong evidence ratio 190.65 from Akaike’s information content criteria analysis as well as extra sum of square F test (P > 0.05), it was found that 3-parameter Andrews model gave the best fit.
Ključne riječi
cutinase; Pseudomonas cepacia; substrate inhibition model; Akaike’s information content criteria; parameter sensitivity
Hrčak ID:
147009
URI
Datum izdavanja:
14.10.2015.
Posjeta: 2.441 *