Original scientific paper
Hydroxycotinine metabolism in rabbit liver, lung and kidney tissue homogenates
M.A. Rashid Mahmud
; Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
A. Nikolin
; Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
B. Nikolin
; Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
M. Lekić
; Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Abstract
Hydroxycotinine metabolism has been investigated in rabbit liver, lung and kidney tissue homogenates, using NADP and NADPH. With NADPH, the liver was the most active organ in metabolizing hydroxycotinine yielding hydroxycotinine-N-oxide (16.3%) as the main metabolic product, along with only little cotinine. With NADP, the formation of cotinine was slightly higher in the kidney and lung tissue incubates than the corresponding formation of cotinine with NADPH. The qualitative identification of the substrate and the metabolic products was carried out by thin-layer chromatography, followed by their quantitative estimation by gas-liquid chromatography.
Keywords
Hrčak ID:
157518
URI
Publication date:
10.10.1984.
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