Original scientific paper
Comparison of spectrophotometric, fluorimetric, high-performance liquid-chromatographic and radioimmunological methods for the determination of theophylline concentrations in biological material
F. Plavšić
; Laboratorij za kliničku farmakologiju, Zavod za kliničku farmakologiju, Kli¬nika za unutrašnje bolesti s poliklinikama Medicinskog fakulteta i Klinički bolnički centar, Zagreb
Abstract
Spectrophotometric, fluorimetric, high-performance liquid chromatographic (HPLC) and radioimmunological (RIA) methods have been introduced for the determination of theophylline levels in the serum. The extraction on charcoal was used for drug isolation from biological fluids before spectrophotometric, fluorimetric and HPLC measurements. For the solid-phase RIA theophylline antibody was covalently bound to CNBr-activated Sepharose. For all introduced methods the inter- and intra-assay coefficients of variation were below 10 per cent in the therapeutic range of concentrations, and the sensitivity was below 1.0 mg. L-1. With the photometric methods high interferences of structurally similar drugs and other materials were observed. The correlations between different methods were good (r = 0.97 and 0.98).
Keywords
Hrčak ID:
158563
URI
Publication date:
29.9.1982.
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