Glasnik Zaštite Bilja, Vol. 39 No. 3, 2016.
Izvorni znanstveni članak
S-SAP molecular marker-based detection of interspecific variability among grape cultivars
Maja Žulj Mihaljević
; Zavod za oplemenjivanje bilja, genetiku i biometriku, Agronomski fakultet, Sveučilište u Zagrebu
Ulrike C. M. Anhalt
; epartment of Crop Sciences, Division of Viticulture and Pomology, University of Natural Resources and Life Sciences, Vienna, Austria
Martina Ramljak
Astrid Forneck
; Department of Crop Sciences, Division of Viticulture and Pomology, University of Natural Resources and Life Sciences, Vienna, Austria
Ivan Pejić
; Zavod za oplemenjivanje bilja, genetiku i biometriku, Agronomski fakultet, Sveučilište u Zagrebu
Sažetak
Intra-varietal variability is based on the assumption that during the time spontaneous mutations occur in the varieties genome and stay fixed because of vegetative propagation. Any kind of information about intra-varietal variability could be of importance before making a decision which vines to keep after verification of their health status and could provide an insight into homogeneity of the population and into the potential of the clonal selection. In this study a retrotransposon based marker system was used – a modified SSAP method which, in previous studies, showed the potential for differentiating clones. Collected grapevine plant material consisted of 22 accessions representing two Croatian varieties: Zlatarica blatska bijela and Glavinuša. S-SAP method was performed using 6 different primer combinations. This method successfully differentiated both varieties and also detected some clonal differences among tested accessions. Although the analyses were not done on certified clonal material, level of polymorphism was similar to those in similar studies using certified clones. The use of universal retrotransposon primer F100 resulted in most observed polymorphism. Obtained results provide guidelines for further propagation and revitalization.
Ključne riječi
clone; intra-varietal variability; retrotransposons; molecular markers; S-SAP
Hrčak ID:
162240
URI
Datum izdavanja:
21.4.2016.
Posjeta: 1.471 *