Veterinary Archives, Vol. 86 No. 5, 2016.
Original scientific paper
The infectious bronchitis vaccine strain virus is more pathogenic in chicken embryos than the wild virus strain 2575/98.
Cheng-Ta Tsai
; School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan
Hue-Ying Chiou
; School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan
Ching-Ho Wang
; School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan
Abstract
An avian infectious bronchitis virus (IBV) strain 2575/98 was attenuated using serial chicken embryo passage to become a vaccine in Taiwan. The aim of this study was to investigate the replication ability, pathogenicity, and tissue tropism of the wild and vaccine strains in chicken embryos. The embryos were inoculated with different titers of wild and vaccine strains. Quantification of virus in allantoic fluid was evaluated using real time RT-PCR. The results showed that the vaccine strain replicated in higher titers than the wild strain, and caused embryo death so quickly that only a few dwarfisms occurred. The embryos inoculated with wild and vaccine strains had similar lesions that were confined primarily to the chorionallantoic membrane (CAM), liver, and kidneys. The immunohistochemical data showed that IBV was present predominantly in the lungs, kidneys, and CAM. Although both strains caused hepatic damage, very few virus antigens were detected in the hepatic tissue. The pathogenicity of the vaccine becomes higher in embryos although it is lower in chickens than its wild strain. The vaccine strain could be used as a possible new vaccine candidate for IBV control.
Keywords
immunohistochemistry; infectious bronchitis; pathogenicity; vaccine
Hrčak ID:
167674
URI
Publication date:
23.9.2016.
Visits: 1.959 *