Izvorni znanstveni članak

Genomic typing and phylogenetic analysis of canine parvovirus detected in the state of Odisha, India.

Monalisa Behera ; Scholar, Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India
Susen K. Panda ; Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India
Sweta Das ; SRF, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India
Pramoda K. Sahoo ; ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India
Aditya P. Acharya ; Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India
Ramesh C. Patra ; Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India
Soumyaranjan Pati ; Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India

Sažetak

Canine parvovirus type 2 (CPV-2) comprises three major antigenic variants CPV-2a, CPV-2b and CPV-2c. Their mutated variants in geographically distinct locations need to be investigated to understand viral evolution and for development of effective management measures. In the present study, 71 faecal and 12 blood samples from suspected dogs in the state of Odisha, India were analyzed by PCR. Faecal lysate, extracted by the fast boiling method was found to be more sensitive as a template for PCR compared to DNA extracted from faecal samples by the phenol-chloroform method. The results revealed 29 positive cases (583 bp amplicon) out of 71 faecal samples, and 5 positive cases out of 12 blood samples examined, with a few variations in the results from blood and faecal samples in the same cases, thus suggesting the necessity of screening both blood and faecal samples for diagnosis. Restriction digestion of the 583 bp PCR amplicon with MboII (PCR-RFLP) confirmed the strain not to be CPV-2c. Further sequencing of the 583 bp fragments recognized the variant as one of the mutated CPV-2a strain. Interestingly, an additional presence of CPV-2a mutant of 525 bp was observed in eleven of the positive faecal samples, along with the 583 bp fragment in PCR that needs further characterization. These two CPV-2a variants shared a common clade with other CPV-2a variants in the phylogenetic tree separating CPV-2b and CPV-2c. Our results confirm the dynamic changes in CPV variants and emphasize the importance of CPV surveillance for understanding of viral epidemiology.

Ključne riječi

canine parvovirus; diagnosis; PCR; new variant

Hrčak ID:

167728

URI

https://hrcak.srce.hr/167728

Datum izdavanja:

23.9.2016.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.752 *