Veterinarski arhiv, Vol. 87 No. 5, 2017.
Izvorni znanstveni članak
https://doi.org/10.24099/vet.arhiv.160430
Post-challenge immune gene expression profiling in rohu, Labeo rohita vaccinated with modified adjuvant-based Aeromonas hydrophila outer membrane protein R formulation
Pujarini Dash
; Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, India
Sunita K. Yadav
; Gene Regulation Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India
Lalit C. Garg
; Gene Regulation Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India
Aparna Dixit
; Gene Regulation Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India
Pramoda K. Sahoo
; Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, India
Sažetak
Although vaccination and post-vaccination studies have gained considerable importance in the field of aquaculture diseases, little is understood about the mechanism of immune-protection in vaccinated fish following infection. In the present study, an attempt was made to discover the mechanism of protection rendered by a modified adjuvant-based recombinant outer membrane protein R (rOmpR) vaccine, in rohu, Labeo rohita (an important Indian major carp species) following a challenge with Aeromonas hydrophila. For this, expression analysis of an array of immune molecules (IgM, complement factor 3, interleukin 1β, interleukin 6, tumor necrosis factor α, interleukin 15, MHC I and MHC II) was carried out in the anterior kidney tissues of vaccinated fish, at 0, 12, 24, 48, 72 and 120 h post-challenge. Higher transcript levels of IgM, MHC I and MHC II were evident in the mineral oil-based OmpR vaccinated and modified adjuvant-based OmpR-vaccinated groups, at different time points post-challenge, including the non-challenged fish. Early onset of pro-inflammatory cytokines and expression of most of the immune genes was more pronounced in vaccinated fish at different time points post-challenge, thus indicating a better immune response in these fish. This study indicated the activation of both humoral and cell mediated immunity in vaccinated rohu following a challenge with A. hydrophila.
Ključne riječi
adjuvant; Aeromonas hydrophila; gene expression; post-challenge; Labeo rohita
Hrčak ID:
186222
URI
Datum izdavanja:
6.9.2017.
Posjeta: 1.842 *