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Regulatory Properties and Cooperativity of Membrane Bound Muscarinic Receptors of Intestinal Smooth Muscle Cells

Dida Kuhnen-Clausen ; Fraunhofer-Gesellschaft, Institut fur Aerobiologie, D 5949 Grafschaft/Sauerland, F. R. G.


Puni tekst: engleski pdf 9.147 Kb

str. 465-474

preuzimanja: 192

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Sažetak

The regulatory properties of the membrane bound muscarinic
acetylcholine receptor (mAChR) of smooth muscles from the guinea
pig ileum are indicated by the S-shaped concentration-response
curve to acetyl-~-methylcholine (MeCh). Cooperativity upon receptor
activation is observed as well as cooperativity of heterotropic
ligands : two agonists, agonist-two antagonists and agonist-antagonist-
interaction. The cooperativity depends either on the integrity
of the cell membrane •structure or of the receptor protein.
Disordering of the membrane structure is caused by a cationic
detergent and results in a loss of sensitivity and cooperativity ·.of
the mAChR for the action of MeCh. Desensitization of the mAChR
results from the blockade of reactive SH-groups, but not from
reduced S-S-bonds. Structural differences between the mAChR
and the nicotinic receptor are discus•sed with respect to essential
disulfide bonds and reactive sulfhydryl groups.

Ključne riječi

Hrčak ID:

196641

URI

https://hrcak.srce.hr/196641

Datum izdavanja:

3.12.1975.

Posjeta: 577 *