Izvorni znanstveni članak
An Extracted Fraction of Pseudomonas Oleovorans Can Inhibit Viral Entry and RNA Replication of Hepatitis C Virus in Cell Culture
Naiera M. H. Mohamed
; Microbial Biotechnology Department, National Research Centre, Dokki, Giza, Egypt
Yasmine El Abd
; Microbial Biotechnology Department, National Research Centre, Dokki, Giza, Egypt
Reem Elshenawy Elshenawy
; Microbial Biotechnology Department, National Research Centre, Dokki, Giza, Egypt
Khaled Zakaria ElBaghdady
; Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt
Abeer A. El-Hadi
; Department of Chemistry of Natural and Microbial Products, National Research Centre,Dokki, Giza, Egypt
Eman A. Ghazy
; Microbial Biotechnology Department, National Research Centre, Dokki, Giza, Egypt
Fawkia M. El-Beeh
; Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt
Robert Smolic
; Faculty of Medicine Osijek, University of Osijek, Osijek, Croatia
Martina Smolic
; Faculty of Medicine Osijek, University of Osijek, Osijek, Croatia
Sažetak
The emergence and distribution of Hepatitis C virus (HCV) infection is still considered as an unsolved problem. Due
to side effects, many synthetic drugs have been avoided and replaced by new biologically derived ones. Aim of this study
was to use Pseudomonas oleovorans’ extract as HCV viral replication inhibition agent in cell culture system. Several factors were studied and the optimum growth conditions were selected for maximum production of antiviral substance.
Pseudomonas oleovorans’ extract was fractionated using different concentrations of chloroform: methanol on silica gel
columns. Analysis of potent fraction by GC/MS showed of tetradecanoic and hexadecanoic acid methyl esters. The selected fraction was tested against HCV in vitro using two different protocols: viral attachment entry inhibition (Pre-incubation) and viral replication inhibition (Post infection). 0.1 µg / ml of the selected antiviral fraction resulted in inhibition
of viral replication in Huh 7.5 cells. However, higher concentration of 100 µg / ml did not cause any viral inhibition. The
selected bacterial fraction containing tetradecanoic acid and hexadecanoic acid methyl esters could be used as a promising candidate to inhibit viral HCV entry and replication of HCV.
Ključne riječi
Pseudomonas oleovorans; Replication; Viral inhibition; HCV and Antiviral
Hrčak ID:
218709
URI
Datum izdavanja:
29.12.2018.
Posjeta: 1.181 *