Kratko priopćenje

https://doi.org/10.2478/acph-2021-0014

A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities

OMAR J. PORTILLO-CASTILLO ; Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, México
ROCÍO CASTRO-RÍOS ; Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, México
ABELARDO CHÁVEZ-MONTES ; Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Química, 66455, Pedro de Alba s/n, Cd. Universitaria, San Nicolás de los Garza, Nuevo León, México
AZUCENA GONZÁLEZ-HORTA ; Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Laboratorio de Ciencias Genómicas, 66455, Pedro de Alba s/n, Cd. Universitaria, San Nicolás de los Garza, Nuevo León, México
NORMA CAVAZOS-ROCHA ; Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, México
NOEMÍ WAKSMAN DE TORRES ; Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, México
MARSELA GARZA-TAPIA ; Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química Analítica, 64460, Francisco I. Madero s/n, Monterrey, Nuevo León, México

Sažetak

Recently, pharmaceutical and personal care products (PPCPs) have received considerable attention because of their increasing use. Analysis of PPCPs presents a significant analytical challenge, with high-performance liquid chromatography (HPLC) in reversed-phase mode, as the most widely used analytical technique. To facilitate the optimization of the procedures that are applied in the early stages of sample preparation, a simple and fast HPLC method is proposed in this work for the separation of some PPCPs with a wide range of hydrophilicity. Two columns were evaluated (Atlantis dC18 and Discovery HS F5); as for mobile phases: a formate buffer (40 mmol L-1, pH 4) and methanol were tested in a gradient mode. The fluorinated column allowed better separation in a shorter time and better resolution for all analytes (Rs >1). The proposed method delivered good performance for the tracing of PPCPs and is a suitable alternative to traditional C18-based HPLC methods.

Ključne riječi

pharmaceutical and personal care products; reversed-phase high-performance liquid chromatography, ultraviolet detection

Hrčak ID:

238372

URI

https://hrcak.srce.hr/238372

Datum izdavanja:

30.6.2021.

Posjeta: 1.187 *