Veterinarski arhiv, Vol. 90 No. 2, 2020.
Izvorni znanstveni članak
https://doi.org/10.24099/vet.arhiv.0563
Genetic characterization, cloning, and expression of Toll-like Receptor 1 mRNA Nile tilapia (Oreochromis niloticus)
Reham R. Abouelmaatti
orcid.org/0000-0001-6777-982X
; Biochemistry Department, Basic Medicine Division, Norman Bethune College of Medical Sciences, Jilin University, Changchun, China; School of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang, China
Abdelazeem M. Algammal
; Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Wael M. K. Elfeil
orcid.org/0000-0001-6777-982X
; Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt; Division of Veterinary Medicine, College of Veterinary Medicine and Animal Science, Jilin University, Changchun, Jilin, China
Noha M. Elshaffy
; Department of Fish Health and Care, Central Lab for Fisheries Research, AL-Abassa, Egypt
Xiaokun Li
; Biochemistry Department, Basic Medicine Division, Norman Bethune College of Medical Sciences, Jilin University, Changchun, China; School of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang, China
Jisheng Ma
; Biochemistry Department, Basic Medicine Division, Norman Bethune College of Medical Sciences, Jilin University, Changchun, China; School of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang, China
Mohamed Fawzy
; Virology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Ali Wahdan
; Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Reham El-Tarabili
; Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Iman I. Shabana
; Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt; Biology Department, Faculty of Science, Taibah University, Al- Madinah Al- Munawara, Saudi Arabia
Sažetak
Toll-like receptors (TLRs) are the most studied group of pathogen recognition receptor categories that detects infectious agents in vertebrates. Fish TLRs exhibit clear, distinct features, structure and a larger diversity than in other vertebrates. This study focused on identifying and detecting the structure of Oreochromis niloticus (Nile tilapia) Toll- like receptor-1 (TLR1|) as a model in freshwater bony fish. The full-length cDNA sequence of Oreochromis niloticus TLR1 mRNA was cloned. Cloning and sequence analysis revealed that the complete cDNA sequence of Oreochromis niloticus TLR1 consists of 2355 base pairs and encodes a polypeptide of 785 amino acids. The molecular analysis of the amino acid sequence indicated that Oreochromis niloticus TLR1 has the standard structural features and major components of amino acids of TLR family members, and is considered an orthologue to the vertebrate TLR1, not a paralogue. The translated amino acid analysis showed 96%, 88%, 85%, and 85% degrees of identity with Zebra Mbuna, Sea bass, Damsel fish, and Clownfish, respectively; and showed 66% identity t with electric eels and 61% with starlets. Phylogenetic analysis revealed that the Nile tilapia TLR1 is closely related to Larimichthys crocea, Epinephelus coioides, and Takifugu rubripes TLR1. Oreochromis niloticus TLR1 was expressed in the kidneys, brain, spleen, intestines, muscle, liver, gills, heart and skin. Quantitative RT-PCR showed differences in the expression levels between the tested tissues. In conclusion, this study is the first report (according to our knowledge) and provides a complete molecular and functional characterization of Oreochromis niloticus toll-like receptor 1, which is considered to be functionally orthologous to TLR1 in other species models.
Ključne riječi
Oreochromis niloticus (Nile tilapia); transmembrane structure; fish; Toll-like receptor; TLR1; fish innate immunity
Hrčak ID:
238615
URI
Datum izdavanja:
25.5.2020.
Posjeta: 1.503 *