Short communication, Note

Studies in the Sphingolipids Series. XI. Synthesis of l-14C-hydroxy-2-aminooctadecane (DL_l- 14C-Sphingine)

D. Keglević ; Tracer Laboratory and Department of Biochemistry, Institute »Ruder Boskovic«, Zagreb, Croatia, Yugoslavia
A. Kisić ; Tracer Laboratory and Department of Biochemistry, Institute »Ruder Boskovic«, Zagreb, Croatia, Yugoslavia
N. Krvavica-Fertić ; Tracer Laboratory and Department of Biochemistry, Institute »Ruder Boskovic«, Zagreb, Croatia, Yugoslavia
M. Proštenik ; Tracer Laboratory and Department of Biochemistry, Institute »Ruder Boskovic«, Zagreb, Croatia, Yugoslavia

Abstract

The preparation of a 14C-labeled sphingine is described. Radioactive carbon was introduced by the treatment of heptadecanoic acid chloride with C-diazomethane, prepared from C-methyl iodide (1 mC). The obtained ketone was converted via 1-C-acetoxy-octadecan-2-one and l-C-hydroxyoctadecan-2-one oxime into 1-14C-hydroxy-2 aminooctadecane (spec. activity: 33,.48 μC /mM). The over-all radiochemical yield based on 14C-methylidide was 12,15%.

Keywords

Hrčak ID:

245374

URI

https://hrcak.srce.hr/245374

Publication date:

28.7.1959.

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