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https://doi.org/10.5513/JCEA01/21.4.2869

Fungal diversity in tomato (Solanum lycopersicum) leaves and fruits in Russia

Lyudmila Kokaeva orcid id orcid.org/0000-0003-1384-3552 ; Moscow Lomonosov State University, Moscow, Leninskiye Gory, 1/12, Russia 119991
Elena Chudinova orcid id orcid.org/0000-0003-3157-494X ; Peoples Friendship University of Russia (RUDN University), Miklukho-Maklaya St., 6, Moscow, Russia 117198
Alexander Berezov orcid id orcid.org/0000-0003-1384-3552 ; Moscow Lomonosov State University, Moscow, Leninskiye Gory, 1/12, Russia 119991
Maria Yarmeeva ; Moscow Lomonosov State University, Moscow, Leninskiye Gory, 1/12, Russia 119991
Peotr Balabko ; Moscow Lomonosov State University, Moscow, Leninskiye Gory, 1/12, Russia 119991
Arseniy Belosokhov ; Moscow Lomonosov State University, Moscow, Leninskiye Gory, 1/12, Russia 119991
Sergey Elansky orcid id orcid.org/0000-0003-1697-1576 ; Moscow Lomonosov State University, Moscow, Leninskiye Gory, 1/12, Russia 119991


Puni tekst: engleski pdf 1.184 Kb

str. 809-816

preuzimanja: 601

citiraj


Sažetak

Sequencing of cloned PCR-amplified species-specific rDNA fragments and isolation of axenic cultures from tomato fruits was carried out to study the mycobiota of tomato leaves and fruits in European part of Russia. DNA was extracted from the leaves, and library of ITS region fragments was constructed in E. coli by cloning of PCR products. This survey revealed fourteen species associated with disease-affected leaves: Septoria lycopersici, Fulvia fulva (=Cladosporium fulvum), Didymella glomerata (=Phoma glomerata), Cladosporium herbarum, Podosphaera fusca, Neocamarosporium goegapense
(=Phoma betae), Rhizoctonia solani, Candida albicans, Dioszegia hungarica, Cladosporium cladosporioides, Didymella lycopersici, Alternaria infectoria, Alternaria alternata, Cryptococcus tephrensis. In the leaves from healthy plants without any visible symptoms DNA of three species was found: Aspergillus versicolor, Alternaria alternata, Aureobasidium pullulans. Analysis of axenic cultures isolated from green diseased tomato fruits revealed fungal species: Alternaria alternata, Alternaria solani,Phomopsis phaseoli, Fusarium equiseti, Chaetomium cochliodes, Clonostachys sp., Irpex lacteus, Colletotrichum coccodes. This research provides new information on the mycobiota of tomato in Southern Russia, the main tomato producing region of the country.

Ključne riječi

mycobiota of tomato leaves; phylloplane; plant pathogenic fungi; fungal species identification; tomato diseases; fungal diversity

Hrčak ID:

248647

URI

https://hrcak.srce.hr/248647

Datum izdavanja:

21.12.2020.

Posjeta: 1.353 *