Stručni rad
THE VALUE OF DETECTION OF PROTEIN P16INK4a AND HPV INFECTION IN CERVICAL INTRAEPITHELIAL NEOPLASIAS AND INVASIVE CARCINOMA OF THE CERVIX
Lidija Pejković
; Privatna ginekološka ordinacija,Solin, Medicinski fakultet Split
Irena Jeličić
; Privatna ginekološka ordinacija,Solin, Medicinski fakultet Split
Snježana Tomić
; Zavod za Patologiju Kliničke bolnice Split
Marko Mimica
; Ginekološka poliklinika »Gynenova« Split
Irena Drmić Hoffman
; Zavod za Patologiju Kliničke bolnice Split
Sažetak
Aim. To establish the existence of a difference between the p16INK4a protein expression in the samples of unchanged, metaplastic and atrophic cervical epithelial tissue and in different levels of epithelial dysplasia and invasive cervical carcinoma, as well as to establish whether there is a connection between the p16INK4a protein expression and the Human Papilloma Virus (HPV) infection, mainly from the high-risk group. Material and methods. The appearance of p16INK4a protein in 50 bioptic samples of cervix has been analysed, out of which 10 samples have been of non-neoplastic cervical epithelial tissue, and 10 samples of each of three cervical dysplasia level, as well as of invasive cervical carcinoma. All samples were previously tested for HPV DNA. Results. Not one of cervical samples with unchanged, metaplastic and atrophic epithelial tissue, contained either HPV infection or a positive staining on p16INK4a protein. Out of 10 samples which had CIN 1 diagnosis, 7 were proven to be HPV infected, and only 2 were positive after the colouring on p16INK4a. Both of these samples were proven to contain high-risk HPV infection (HR HPV). Out of 10 samples which had CIN 2 diagnosis, 7 were proven to contain HR HPV and in all of these samples a positive p16INK4a protein expression was found. In two out of three samples which were not proven to be HPV infected, there was a positive staining on p16INK4a protein. Analysing the samples which had CIN 3 diagnosis, HR HPV was found in 8, and in all of these samples a positive p16INK4a protein expression was found. In one out of two HPV negative samples a positive staining on p16INK4a protein was found. In the analysis of 10 samples with invasive cervical carcinoma diagnosis, in 7 containing HR HPV a positive staining on p16INK4a protein was found. A positive staining on p16INK4a protein was also found in two out of three HPV negative samples. Conclusion. The p16INK4a protein does not appear in the epithelial tissue without dysplasia changes, and the frequency of its appearance increases with the dysplasia grade. With the increase in the dysplasia level, the frequency of HR HPV infection gets higher. The p16INK4a protein is a good virus-infection indicator, although there are cases of this protein’s appearance without the infection. According to the results of this research, immunohistochemical colouring on p16INK4a protein shows its value in the cytological and bioptical cervical samples’ interpretation. This is a preliminary work and displays the results of the majority of the incoming research.
Ključne riječi
HPV; p16INK4a; cervical dysplasia
Hrčak ID:
23524
URI
Datum izdavanja:
1.3.2008.
Posjeta: 3.058 *