Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2020.1864
Model-based Optimization of Biopolymer Production from Glycerol
K. Sharma
; Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India
A. K. Srivastava
; Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India
T. R. Sreekrishnan
; Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India
Sažetak
The present study focuses on sustainable production of biodegradable polymers by Cupriavidus necator DSMZ 545 using glycerol as substrate. The batch growth and biopolymer production kinetics were established in a 7-L bioreactor, which resulted in a total biomass of 8.88 g L–1 and poly(3-hydroxybutyrate) (PHB) accumulation of 6.76 g L–1. The batch kinetic and independently acquired substrate inhibition data were then used to develop a mathematical model for PHB production process. This was eventually used to design different nutrient feeding strategies under constant feed rate, decreasing feed rate, and pseudo steady state of substrate (glycerol) to optimize the PHB production during fed-batch cultivation. Among all the fed-batch cultivation strategies, the highest PHB accumulation and productivity of 13.12 g L–1 and 0.27 g L–1 h–1, respectively, was achieved in fed-batch bioreactor cultivation where a pseudo steady state with respect to glycerol was maintained.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
poly(3-hydroxybutyrate); Cupriavidus necator; glycerol; mathematical model; fed-batch; cultivation strategies
Hrčak ID:
256423
URI
Datum izdavanja:
25.4.2021.
Posjeta: 772 *