Veterinary Archives, Vol. 91 No. 3, 2021.
Original scientific paper
https://doi.org/10.24099/vet.arhiv.1004
Molecular identification and phylogenetic analysis of Trypanosoma evansi in dromedaries (Camelus dromedarius) from Iran
Somayeh Bahrami
; Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Golestan Boulevard, Ahvaz, Khuzestan Province, Iran
Ali R. Alborzi
; Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Golestan Boulevard, Ahvaz, Khuzestan Province, Iran
Saeid Rahimi Esfahsalari
; Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Golestan Boulevard, Ahvaz, Khuzestan Province, Iran
Zahra Ziafati
; Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Azadi Av.Tehran, Tehran Province, Iran
Abstract
Surra is of great concern to countries in the world such as Iran, which have a considerable camel population. The present study was aimed at determining the prevalence of Trypanosoma evansi in the camels of Iran. A total of 167 blood samples from farmed camels were examined for the presence of T. evansi infection using parasitological and molecular methods. Blood smear examination revealed 10 (6%) positive samples, while the PCR method 14 (8.4%) found positive samples. Age, sex, and region were not determined as risk factors for T. evansi infection in this study. The phylogenetic tree inferred from VSG gene sequences of T. evansi clearly separated the sequences of this study into two clades, A and B, which reflects the intrasequence heterogeneity among Iranian isolates. The phylogenetic tree showed that Iranian T. evansi strains are members of the T. brucei clade.
Keywords
Trypanosoma evansi; camel; Iran; phylogenetic tree
Hrčak ID:
260231
URI
Publication date:
9.7.2021.
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