Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2021.1921
Biosynthesis of γ-Polyglutamic Acid by Bacillus licheniformis Through Submerged Fermentation (SmF) and Solid-state Fermentation (SSF)
V. Campos
; São Paulo State University (Unesp), Institute of Science and Technology, Sorocaba/SP/Brazil
R. M. Kaspary
; São Paulo State University (Unesp), Institute of Science and Technology, Sorocaba/SP/Brazil
M. J. Rossi
; Federal University of Santa Catarina, Department of Microbiology, Immunology and Parasitology, Florianópolis/SC/Brazil
T. A. M. Medeiros
; São Paulo State University (Unesp), Institute of Science and Technology, Sorocaba/SP/Brazil
Sažetak
Gamma-Polyglutamic acid (γ-PGA) was produced from fermentation processes by inoculating the bacterium Bacillus licheniformis on different substrates. In submerged
fermentation (SmF), glycerol and glucose were used as the main carbon substrates while ammonium sulfate served to provide nitrogen. In solid-state fermentation (SSF), soybean was used as the main substrate. Tests carried out in an airlift bioreactor with B. licheniformis showed a maximum productivity of 0.789 g L–1 h–1 and a yield of 0.4 g g–1. Different soybean cultivars from the 2015 and 2016 crops were used in the SSF. The BRS 1001IPRO cultivar, from the 2016 crop, showed the highest production, i.e., 1.2 g L–1 of γ-PGA. This cultivar was subjected to different levels of milling (fine, intermediate and coarse). Coarsely milled grains increased γ-PGA production to 2.7 g L–1. Moreover, coarse milling made the internal nutrients of the grains available for fermentation and this particle size did not compromise aeration during fermentation.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
γ-polyglutamic acid; biosynthesis; B. licheniformis; SmF; SSF
Hrčak ID:
264351
URI
Datum izdavanja:
20.10.2021.
Posjeta: 1.224 *