Veterinarski arhiv, Vol. 77 No. 3, 2007.
Izvorni znanstveni članak
Rapid detection of Mycobacterium bovis in clinical samples using thp-1 cells
Manoharan Parthiban
; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Rengarajan Jaiganesh
; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Wilson Santhosh Kumar Aruni
; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Sundaram Meignanalakshmi
; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Pachikani Ramadass
; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Sažetak
The rapid isolation of M. bovis was attempted in THP-1 cells (Macrophage cell line). THP-1 cells infected with M. bovis showed characteristic cytopathic effect 48 hr after infection. The bacteria had grown very well in the first passage itself, without any blind passages in cell culture. The presence of bacteria in the cell culture fluid was confirmed by Ziehl-Neelsen staining and nested PCR amplification of insertion sequences.
Ključne riječi
M. bovis; THP-1 cells; nested PCR; Ziehl-Neelsen stain; decontamination
Hrčak ID:
24847
URI
Datum izdavanja:
20.6.2007.
Posjeta: 1.915 *