Skoči na glavni sadržaj

Izvorni znanstveni članak

https://doi.org/10.5562/cca3830

Comparison of Zn Content in Rapid-acting Insulin and Biphasic Suspension by FAAS

Sofia Shkunnikova orcid id orcid.org/0000-0003-2546-0823 ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, Zagreb, Croatia
Tea Širac ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, Zagreb, Croatia
Katarina Lalić ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, Zagreb, Croatia
Ankica Kulišić ; University Hospital Center Zagreb, Kišpatićeva 12, Zagreb, Croatia
Jasna Jablan orcid id orcid.org/0000-0003-4741-0819 ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, Zagreb, Croatia


Puni tekst: engleski pdf 1.338 Kb

str. P1-P8

preuzimanja: 75

citiraj


Sažetak

Various insulin analogs (rapid-acting and intermediate-acting insulin) have been investigated for the determination of zinc content by flame atomic absorption spectrometry. This paper presents the validation of a method and comparison of zinc content in the insulin samples studied. The method was linear \(r\)\(^2\) = 0.9997), the limit of detection was 0.0098 mg L\(^{–1}\) and the limit of quantification was 0.0296 mg L\(^{–1}\), the precision (as relative standard deviation) was up to 7.4 %, and the accuracy was within a range of 95.6 % to 100.1 % for the recovery of fortified insulin samples. The zinc content in the insulin samples ranged from 14.9 mg L\(^{–1}\) to 16.3 mg L\(^{–1}\) for rapid-acting insulin and 18.7 mg L\(^{–1}\) to 19.9 mg L\(^{–1}\) for intermediate-acting insulin. A higher zinc content was found in the intermediate-acting insulins than in the rapid-acting insulin analogs (\(p\) < 0.05). The obtained results should be considered in the establishment of new or improvement of currently available procedures used to assure the quality, safety and efficacy of insulin products.

This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi

zinc, rapid-acting and intermediate-acting insulins, flame atomic absorption spectrometry

Hrčak ID:

271136

URI

https://hrcak.srce.hr/271136

Posjeta: 210 *