Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2021.62.250
Uroguanylin increases Ca2+ concentration in astrocytes via guanylate cyclase C-independent signaling pathway
Nikola Habek
; Laboratory of Cellular Neurophysiology, Croatian Institute for Brain Research, Zagreb University School of Medicine, Zagreb, Croatia
Martina Ratko
; Center of Excellence for Basic, Clinical and Translational Neuroscience, Zagreb University School of Medicine, Zagreb, Croatia
Aleksandra Dugandžić
orcid.org/0000-0001-9454-7207
; Laboratory of Cellular Neurophysiology, Croatian Institute for Brain Research, Zagreb University School of Medicine, Zagreb, Croatia
Sažetak
Aim To investigate the cyclic guanosine monophosphate
(cGMP)/guanylate cyclase C (GC-C)-independent signaling
pathway in astrocytes, which are a suitable model due to
their lack of GC-C expression.
Methods Patch clamp was performed and intracellular
Ca2+ concentrations and pH were measured in primary
astrocyte cultures and brain slices of wild type (WT) and
GC-C knockout (KO) mice. The function of GC-C-independent signaling pathway in the cerebellum was determined
by behavior tests in uroguanylin (UGN) KO and GC-C KO
mice.
Results We showed for the first time that UGN changed
intracellular Ca2+ levels in different brain regions of the
mouse. In addition to the midbrain and hypothalamus,
GC-C was expressed in the cerebral and cerebellar cortex.
The presence of two signaling pathways in the cerebellum (UGN hyperpolarized Purkinje cells via GC-C and increased intracellular Ca2+ concentration in astrocytes) led
to a different motoric function in GC-C KO and UGN KO
mice, probably via different regulation of intracellular pH
in astrocytes.
Conclusion The UGN effects on astrocytes via a Ca2+-dependent signaling pathway could be involved in the modulation of neuronal activity.
Ključne riječi
Hrčak ID:
278134
URI
Datum izdavanja:
24.6.2021.
Posjeta: 391 *