Izvorni znanstveni članak

https://doi.org/10.46419/vs.54.2.1

Conventional and functional evaluation of semen in male dairy goats

Daniela Herrera Vargas ; Semillero de Investigación y Grupo de Estudio en Caprinos y Ovinos (GECO-GaMMA), Facultad de Ciencias Agrarias, Universidad de Antioquia. Medellín, Colombia
Maricela Castaño Escobar ; Semillero de Investigación y Grupo de Estudio en Caprinos y Ovinos (GECO-GaMMA), Facultad de Ciencias Agrarias, Universidad de Antioquia. Medellín, Colombia
Daniela Arcila Davila ; Semillero de Investigación y Grupo de Estudio en Caprinos y Ovinos (GECO-GaMMA), Facultad de Ciencias Agrarias, Universidad de Antioquia. Medellín, Colombia
Jorge López Pérez ; Empresa de Biotecnología Reproductiva, Teriogén S.A.S, Medellín, Colombia
Jose Manuel Mayorga-Torres ; Grupo Reproducción, Facultad de Medicina, Universidad de Antioquia. Medellín, Colombia
Walter D. Cardona-Maya ; Grupo Reproducción, Facultad de Medicina, Universidad de Antioquia. Medellín, Colombia
Henry Cardona Cadavid ; Grupo de Investigación en Genética, Mejoramiento y Modelación Animal (GaMMA), Universidad de Antioquia, Medellín, Colombia

Sažetak

Evaluating the reproductive indicators in sheep breeding in Colombia, particularly males, is essential to achieve optimal production parameters. This study aimed to evaluate the semen quality of male goats through both conventional and functional seminal analysis. Semen samples from eight goats were collected and conventional (volume, appearance, colour, motility, vigour, and morphology) and functional (plasma membrane integrity, mitochondrial membrane potential, DNA integrity, lipid peroxidation, and reactive oxygen species production) seminal parameters were evaluated. The results showed an average scrotal circumference of 25.69 cm, seminal volume of 0.51 mL, and concentration of 1936 x 106 sperm/mL. The average individual motility was 63.37%, vigour was four, and the percentage of abnormalities was 7.75%. Individual motility was correlated with the mitochondrial membrane potential (r=0.840, P=0.009) and reactive oxygen species production (r=-0.91, P=0.001). The average of cells with high mitochondrial membrane potential was 52.94%, while 39.29% were necrotic cells, the DNA fragmentation index average was 12.5%, reactive oxygen species production was 38.68%, lipoperoxidation analysis was 7.33% on average, and the integrity of the membrane was 54.77%. The results establish the parameters for the semen from Antioquia goats and confirm that goat semen is of good quality and could be used during artificial insemination programs.

Ključne riječi

sperm; goat; reproduction; flow cytometry; semen

Hrčak ID:

279022

URI

https://hrcak.srce.hr/279022

Datum izdavanja:

14.8.2022.

Podaci na drugim jezicima: hrvatski

Posjeta: 655 *