Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2021.2038
Separation of Crocin/Betanin Using Aqueous Two-phase Systems Containing Ionic Liquid and Sorbitol
R. Madadi
orcid.org/0000-0003-1732-1372
; Department of Food Science and Technology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
Sh. Shahriari
orcid.org/0000-0002-6922-2993
; Department of Chemical Engineering, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
H. Mozafari
orcid.org/0000-0002-6533-7400
; Department of Agronomy, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
Sažetak
Betanin and crocin, two food additives with attractive colors, are bioactive compounds of plants that are widely used in food, pharmaceutical,
and medical industries. These bioactive pigments are sensitive to light, heat, organic solvents, and pH. It seems that a benign economic method is needed to extract these biomolecules, especially for industrial applications. The aqueous two-phase system (ATPS) is a liquid-liquid extraction technique that has shown its potential in recent years to extract and separate biomolecules. In this study, an ATPS consisting of carbohydrate (sorbitol) and ionic liquid (tetrabutyl phosphonium bromide) has been proposed as a new separation system with unique properties to study the partition coefficient of crocin and betanin. The results indicated that crocin and betanin had more tendency to the ionic liquid (IL)-rich phase and carbohydrate-rich phase, respectively. The influence of the concentration of IL and sorbitol on the partition coefficient was studied. The results showed that an increase in the tie-line length (concentrations) increased the partition coefficient of crocin and betanin. Enhancement in temperature increased the partition coefficient of crocin. The highest values of crocin recovery (97.55 %) and partition coefficient (39.85) at 308 K show that our proposed ATPS can be considered for crocin separation in one step.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
ionic liquid; carbohydrate; crocin; betanin; extraction; aqueous two-phase systems
Hrčak ID:
280939
URI
Datum izdavanja:
26.7.2022.
Posjeta: 741 *