Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2021.1967
Byproduct from Triphala Extraction as Tannin and Rutin Sources for Production of Gallic Acid, Isoquercetin and Quercetin by Solid-State Fermentation
T. Chysirichote
orcid.org/0000-0001-8358-5769
; Department of Food Engineering, School of Engineering, King Mongkut’s Institute of Technology Ladkrabang, 1 Chalongkrung 1, Chalongkrung Rd., Ladkrabang, Bangkok 10520 Thailand
P. Pakaweerachat
; Department of Food Engineering, School of Engineering, King Mongkut’s Institute of Technology Ladkrabang, 1 Chalongkrung 1, Chalongkrung Rd., Ladkrabang, Bangkok 10520 Thailand
Sažetak
Byproduct from Triphala extraction process (BTP) was studied as a substrate for gallic acid, isoquercetin and quercetin production by Aspergillus niger
fermentation in this research. The results showed that BTP was a good source of tannin and rutin. Nevertheless, the activity of A. niger on BTP as a sole substrate was very low. Supplementing nitrogen sources was found to be a key to enhancing conversion of tannin to gallic acid, and rutin to isoquercetin or isoquercetin and quercetin. BTP with 0.75 % sodium nitrate was suggested to be an optimal supplemented nitrogen source for the production of gallic acid and isoquercetin in this research, which yielded the highest contents of 61.6±2.16 mg g–1 DS and 3.27±0.29 mg g–1 DS, respectively. In addition, the highest extraction yields of gallic acid, isoquercetin and quercetin were obtained by an ultrasound-assisted extraction using methanol as an extraction solvent as 12.24±2.12 mg g–1 DS which was around 0.5 time higher than the one without ultrasound-assisted extraction (8.84±1.12 mg g–1 DS).
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
triphala; solid-state fermentation; gallic acid; isoquercetin; extraction
Hrčak ID:
280942
URI
Datum izdavanja:
26.7.2022.
Posjeta: 472 *