Izvorni znanstveni članak
Genotoxic effects of irinotecan and cisplatin on the healthy cells of mice evaluated by alkaline comet assay
Gordana Brozović
; Department of Anesthesiology and ICU, University Hospital for Tumors, Zagreb, Croatia
Nada Oršolić
; Department of Animal Physiology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Fabijan Knežević
; Department of Pathology, University Hospital for Tumors, Zagreb, Croatia
Anica Horvat-Knežević
; Department of Animal Physiology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Katarina Šakić
; Department of Anesthesiology, Reanimatology and Intensive Medicine, Clinical Medical Center Zagreb, Zagreb, Croatia
Vesna Benković
; Department of Animal Physiology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Danko Velimir Vrdoljak
; Department of Surgical Oncology, University Hospital for Tumors, Zagreb, Croatia
Sažetak
The purpose of cytostatic agents is to act exclusively upon tumor cells, and to inhibit growth or induce tumor cell death by impairing their cell cycle progression. However, the majority of these agents are not specific in their action, and subsequently produce toxic effects on healthy tissues causing significant adverse events in both patients and health professionals exposed to these drugs. Various cytogenetic and molecular biology assays play an important role in the assessment of genotoxic effects related to antineoplastic drugs. Within a short period after exposure to a potentially genotoxic agent, these assays are able to assess the level of cellular DNA damage and/or to monitor the dynamics of DNA repair. Sensitive techniques, such as alkaline comet assay, are of special importance in the detection of primary DNA damage occurring in individual cells regardless of the cell cycle phase. The aim of the study was to assess and compare DNA damage that irinotecan and cisplatin induce in peripheral leukocytes, and normal kidney, liver and brain cells of Swiss albino mice. The results show that both cytostatics produce statistically significant DNA damage in normal cells compared to the control group. Compared to irinotecan, cisplatin has a significantly more potent genotoxic effect on these cells, which may be attributed to various mechanisms of action of the studied drugs.
Ključne riječi
genotoxicity; irinotecan; cisplatin; mice; alkaline comet assay
Hrčak ID:
281518
URI
Datum izdavanja:
30.11.2005.
Posjeta: 328 *