Medicina, Vol. 44 No. 1, 2008.
Izvorni znanstveni članak
Expression of bone morphogenetic protein -2 and -7 during experimental inflammatory bowel disease
Ivana Marić
orcid.org/0000-0001-9318-5726
; Zavod za anatomiju, Medicinski fakultet Sveučilišta u Rijeci, Hrvatska
Natalia Kučić
; Zavod za fiziologiju i imunologiju
Blaženka Grahovac
; Zavod za patologiju i patološku anatomiju
Dragica Bobinac
; Zavod za anatomiju, Medicinski fakultet Sveučilišta u Rijeci, Hrvatska
Slobodan Vukičević
; Zavod za anatomiju, Medicinski fakultet Sveučilišta u Zagrebu, Hrvatska
Sažetak
ABSTRACT. Aim: Bone morphogenetic proteins -2 and -7, along with their osteoinductive
function, play important role in the development and healing of different non-skeletal tissues
and organs. The aim of this study was to reveal the expression of BMP-2 and BMP-7 during
experimental inflammatory bowel disease, as well as after 14th day of BMP-7 treatment.
Methods: Experimental colitis was induced by the intracolonic administration of the clysma
containing 2,4,6-trinitrobenzensulphonic acid (TNBS) and ethanol. We investigated the
macroscopic and microscopic colon tissue damage during acute and chronic phases of colitis as
well as after IV treatment of BMP-7. Results: Imunohistochemical and RT-PCR analyses showed
expression of BMP-2 and -7 during acute and chronic phases of inflammatory bowel disease. In
the acute phase of colitis, BMP-2 and -7 expressions were located in mucosa of ulcer region. In
the chronic phase BMP-7 expression was placed in the colonocytes along the crypt, while BMP-2
expression was located in the coloncytes of surface epithelium and intercrypt table. BMP-7
treatment reduced the expression of BMP-7 and mRNA for BMP-7 but also increased the
expression of BMP-2 and mRNA for BMP-2 in colonic tissue. Conclusions: Systemic administration
of bone morphogenetic protein 7 decreased macroscopic and microscopic colonic tissue damage
during experimental inflammatory bowel disease. The expression pattern of BMP-2 and -7 during
inflammatory bowel disease, as well as after BMP-7 treatment suggests an important role of
bone morphogenetic proteins in control of colon tissue damage and healing during disease.
Ključne riječi
bone morphogenetic protein-2; bone morphogenetic protein -7; immunohistochemistry; inflammatory bowel disease; polymerase chain reaction
Hrčak ID:
25952
URI
Datum izdavanja:
1.6.2008.
Posjeta: 1.734 *