Izvorni znanstveni članak
Ultrastructural Changes, Nuclear Factor-κB Activation, and Tumor Necrosis Factor-α Expression in Brain after Acute Normovolemic Hemodilution and Controlled Hypotension in Rats
Ran Lv
; Klinika za Anesteziologiju, Medicinskog fakulteta, Zhejiang, Hangzhou, Kina
Wei Zhou
; Klinika za opću kirurgiju, Medicinskog fakulteta, Zhejiang, Hangzhou, Kina
Manlin Duan
; Klinika za Anesteziologiju, Medicinskog fakulteta, Zhejiang, Hangzhou, Kina
Yali Ge
; Klinika za Anesteziologiju, Medicinskog fakulteta, Yangzhou, Kina
Taidi Zhong
; Klinika za Anesteziologiju, Medicinskog fakulteta, Zhejiang, Hangzhou, Kina
Sažetak
Aim To examine brain damage following different degrees of acute
normovolemic hemodilution combined with controlled hypotension
(ANH-CH) by neuronal morphological analysis and investigate the expression
of nuclear factor-kappa B (NF-κB) activity and tumor necrosis
factor-alpha (TNF-α) in the rat.
Methods Forty rats were randomly assigned to receive a sham operation
or ANH-CH (with hematocrit 30%, 25%, 20%, and 15%). ANH
was performed after baseline physiological parameters had been monitored
for 20 minutes. CH was induced 30 minutes later using sodium
nitroprusside and mean arterial pressure was maintained at 50-60 mm
Hg for 1 hour. Rats were euthanatized 3 and a half hours after operation.
TNF-α levels and NF-κB activities in cerebral temporal cortex
were measured. Ultrastructural alterations in the CA1 region of the rat
hippocampi were observed. Changes in mitochondria were evaluated
semiquantitatively.
Results Marked ultrastructural alterations, such as mitochondrial denaturalization
and nucleus distortion, were observed in the CA1 region
of the hippocampus in the ANH-CH hematocrit 20% group and
ANH-CH hematocrit 15% group. TNF-α expression and NF-κB activity
in the cerebral temporal cortex significantly increased in all ANHCH
groups and peaked in the ANH-CH hematocrit 25% group.
Conclusion Severe ANH-CH with hematocrit ≤20% may induce cerebral
damage and should be avoided. NF-κB activation and TNF-α
expression may play a functional role under the ischemic condition. A
better understanding of the role of NF-κB and TNF-α in the brain may
lead to a novel approach for preventing and treating various neurological
disorders.
Ključne riječi
hemodilution; hypotension, controlled; brain damage; hippocampus; TNF-α; NF-kappa B
Hrčak ID:
26086
URI
Datum izdavanja:
15.2.2008.
Posjeta: 1.320 *