Veterinarska stanica, Vol. 54 No. 6, 2023.
Izvorni znanstveni članak
https://doi.org/10.46419/vs.54.6.4
Molecular Phylogeny and Characterization of Mundri Sheep (Ovis aries) of Pakistan through Sequencing of Mitochondrial Cytochrome b and Cytochrome Oxidase Subunit I
Tuba Noreen
; Department of Zoology, University of Education, Lahore, Pakistan
Tanveer Hussain
; Department of Molecular Biology, Virtual University of Pakistan, Rawalpindi, Pakistan
Muhammad Mansha
; Department of Biotechnology, Virtual University of Pakistan, Lahore, Pakistan
Zinnia Mansoor
; Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, Balochistan, Pakistan
Abdul Wajid
; Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, Balochistan, Pakistan
Muhammad Rizwan Ashraf
; Department of Biotechnology, Virtual University of Pakistan, Lahore, Pakistan
Goher Ayub
; Department of Biotechnology, Virtual University of Pakistan, Lahore, Pakistan
Muneeb M. M. Musthafa
; Department of Biosystems Technology, Faculty of Technology, Southeastern University of Sri Lanka, Sri Lanka
Faiz M. M. T. Marikar
orcid.org/0000-0003-4579-7263
; General Sir John Kotelawala Defense University, Ratmalana, Sri Lanka
Sažetak
The main focus of this research is to determine the molecular phylogeny and characterization of Mundri Sheep (Ovis aries) through sequencing of mitochondrial Cytochrome b and Cytochrome Oxidase Subunit I (COI). This sheep breed appears morphologically different from other local sheep breeds of Pakistan. The current research is carried out to appraise the status of Mundri sheep whether it is aa different breed from other breeds or not. Blood samples of Mundri sheep were collected from Livestock Experiment Station, (LES) Fazilpur in district Rajanpur (Punjab). DNA was isolated and subjected to Polymerase Chain Reaction (PCR) for amplification of Cytochrome b and COI genes using appropriate primers. PCR products were sequenced and analyzed by MEGA X software. The phylogeny analysis categorized Ovis aries including Mundri sheep into three and two groups for Cytochrome b and COI genes respectively. It showed Mundri sheep as a separate group and thus as a separate breed from all other local sheep breeds. Hence the study validates based on Cytochrome b and COI that Mundri sheep is a distinctive breed from the rest of the local sheep breed.
Ključne riječi
Mundri Sheep; Ovis aries; Molecular Phylogeny; Characterization; Cytochrome b; Cytochrome Oxidase Subunit I; COI; Pakistan
Hrčak ID:
291378
URI
Datum izdavanja:
19.5.2023.
Posjeta: 381 *