Original scientific paper

Laser direct biphotonic photolysis of guanines within DNA

Z. Shaqiri ; Institute of Solid State Physics, Bulgarian Academy of Sciences, Tzargradsko Chaussee blvd. 72, BG-1784, Sofia, Bulgaria
A. Veseli ; Faculty of Natural and Mathematical Sciences, 38000, Prishtina, Kosovo
N. Shaqiri ; Academy of Medicine blvd. 1, G. Sofiski, Sofia, Bulgaria
E. Vidolova-Angelova ; Institute of Solid State Physics, Bulgarian Academy of Sciences, Tzargradsko Chaussee blvd. 72, BG-1784, Sofia, Bulgaria
D. Angelov ; Institute of Solid State Physics, Bulgarian Academy of Sciences, Tzargradsko Chaussee blvd. 72, BG-1784, Sofia, Bulgaria

Abstract

Nanosecond laser-induced oxidative lesions at guanines within DNA were investigated using sequencing gel electrophoresis analysis. Strong sequence-specific lesions on guanines were revealed by either Fpg or piperidine treatments and assigned to 8-oxoG and oxazolone, respectively. It was shown that both the biphotonic ionization process and the chemical reactivity of the radical cation (G+) are dependent on the DNA sequence. The former were explained by the occurrence of energy and charge migration phenomena, while the latter in terms of local DNA hydration peculiarities.

Keywords

Fpg; piperidine; laser photolysis; 8-oxoG; oxazolone; guanine radical; energy and charge transfer in DNA

Hrčak ID:

301594

URI

https://hrcak.srce.hr/301594

Publication date:

1.10.2002.

Article data in other languages: croatian

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