Original scientific paper

https://doi.org/10.24099/vet.arhiv.2146

Novi VP2/VP3 rekombinantni senekavirus A izoliran u sjevernoj Kini

Yingru Ma ; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China
Limei Zheng ; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China
Chen Li ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Xiaoyan Wu ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Jianli Shi ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Chang Liu ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Hong Han ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Shaojian Xu ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Nataliia Hrabchenko ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Mengjie Huang ; College of Animal Science and Veterinary Medicine Shandong Agricultural University, Taian, China
Jianhua Qiu ; College of Animal Science and Veterinary Medicine Shandong Agricultural University, Taian, China
Jun Li ; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Xianjie Han ; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China

Abstract

Senekavirus A (SVA), prije nazivan virusom doline Seneca Valley, jedini je pripadnik roda senekavirusa u porodici
Picornaviridae. Virus je slučajno otkriven 2002. i nazvan virusom doline Seneca 001 (SVV-001). SVA je novi patogen
koji može uzrokovati vezikularne lezije i prolaznu epidemiju novorođene prasadi s naglim gubicima u proizvodnji. U
ovom je istraživanju soj SVA izoliran u populaciji svinja iz provincije Shandong u Kini i identificiran kao SVA-CHSDFX-2022. Kompletni genom izolata SVA imao je 7282 nukleotida (nt) u dužini i sadržavao je jedan otvoreni okvir
za očitavanje (ORF), bez poli-A repova. Filogenetska je analiza pokazala da izolat u velikoj mjeri sadržava genomsku
organizaciju i nukleotidne identitete, od 90,5 % do 99,6 %, s drugim poznatim SVA izolatima. Karakterizacija virusa
je pokazala da ima veliku sposobnost rasta in vitro. Pronađena je rekombinacija izolata SVA-CH-SDFX-između
nukleotida 1836 i 2710 što je uključilo regiju gena VP2 (parcijalno) i gena VP3 (parcijalno). Zbog visoke stope
infektivnosti i golemih ekonomskih gubitaka važan je brži razvoj cjepiva i bolje razumijevanje zaraze. Rezultati ovog
istraživanja pružaju korisne podatke za proučavanje SVA virusa, posebno s obzirom na njegovu epidemiologiju u
svinja i regionalnu prekograničnu kontrolu vezikularnih bolesti.

Keywords

senekavirus A; svinje; izolacija virusa; IFA; evolucijska analiza; Western blotting

Hrčak ID:

305599

URI

https://hrcak.srce.hr/305599

Publication date:

25.6.2023.

Article data in other languages: english

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