GAZETTE FUTURE, Vol. 6 No. 2-3, 2023.
Original scientific paper
https://doi.org/10.32779/gf.6.2-3.2
Phenolic compounds in Geranium dalmaticum (Beck) Rech. f. and G. macrorrhizum L. (Geraniaceae) growing in Croatia
Dario Kremer
orcid.org/0000-0002-4050-4575
; University of Zagreb, Faculty of Pharmacy and Biochemistry, Pharmaceutical Botanical Garden "Fran Kušan", Zahreb.
Iztok Jože Košir
orcid.org/0000-0002-2829-1335
; Slovenian Institute of Hop Research and Brewing, Žalec, Slovenia.
Marina Šimunić
; University of Zagreb, Faculty of Agriculture, Zagreb.
Ksenija Karlović†
; University of Zagreb, Faculty of Agriculture, Zagreb.
Siniša Srečec
orcid.org/0000-0002-9009-4375
; Križevci College of Agriculture, Križevci,
Renata Jurišić Grubešić
; University of Rijeka, Faculty of Medicine, Rijeka.
Abstract
The quantitative analysis of bioactive phenolic compounds was carried out for the first time in aboveground plant parts of Geranium dalmaticum (Beck) Rech. f. and G. macrorrhizum L. (Geraniaceae) from Croatia by means of UV-Vis spectrophotometric methods and HPLC analysis. Both species had similar contents of polyphenolic substances expressed as mg of standard equivalent /g of dried herbal sample: total polyphenols (217.60 ± 1.08 and 215.53 ± 1.10 mg/g), tannins (155.83 ± 0.60 and 157.73 ± 0.61 mg/g), total flavonoids (5.10 ± 0.10 and 5.53 ± 0.10 mg/g), and total phenolic acids (13.27 ± 1.34 and 15.33 ± 0.45 mg/g), respectively for G. dalmaticum and G. macrorrhizum. Only the content of phenolic acids was somewhat higher in G. macrorrhizum but it was not statistically significant (p > 0.05). HPLC analysis showed that, among seven tested phenolic compounds, only quercetin was quantified in G. dalmaticum (0.23 % of dry weight), and rutin in G. macrorrhizum (1.12 % of dry weight). The obtained results represent a useful basis for further research of phytochemicals and biological activities of G. dalmaticum and G. macrorrhizum extracts.
Keywords
Geranium, eastern Adriatic, polyphenols, flavonoids, phenolic acids.
Hrčak ID:
306886
URI
Publication date:
30.6.2023.
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