Izvorni znanstveni članak
https://doi.org/10.2478/acph-2023-0036
Functionalization of selenium nanoparticles with olive polyphenols – impact on toxicity and antioxidative activity
EMERIK GALIĆ
KRISTINA RADIĆ
; University of Zagreb Faculty of Pharmacy and Biochemistry, 10 000 Zagreb, Croatia
NIKOLINA GOLUB
; University of Zagreb Faculty of Pharmacy and Biochemistry, 10 000 Zagreb, Croatia
JAKOV MLINAR
DUBRAVKA VITALI ČEPO
; University of Zagreb Faculty of Pharmacy and Biochemistry, 10 000 Zagreb, Croatia
TOMISLAV VINKOVIĆ
; Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, 31 000 Osijek, Croatia
Sažetak
Selenium nanoparticles (SeNPs) represent novel selenium (Se) formulation characterized by improved biocompatibility and a wider therapeutic range in comparison to inorganic Se. The aim of this work was to investigate the possibilities of functionalization of SeNPs with olive pomace extract (OPE), rich in health-promoting polyphenols, and to obtain innovative forms of nutraceuticals. Cytotoxic and antioxidative activities of four types of SeNPs (polyvinylpyrrolidone stabilized (PVP SeNPs), polysorbate stabilized (PS SeNPs), polyvinylpyrrolidone stabilized and functionalized using OPE (f PVP SeNPs) and polysorbate stabilized and functionalized using OPE (f PS SeNPs) were investigated. SeNPs showed lower toxicity on human hepatocellular carcinoma (HepG2) and human colorectal adenocarcinoma (Caco2) cells compared to selenite. Functionalization with polyphenols significantly improved their direct antiradical (f PVP SeNPs: 24.4 ± 1.84 and f PS SeNPs: 30.9 ± 2.47 mg TE/mmol Se) and reducing properties (f PVP SeNPs: 50 ± 3.16 and f PS SeNPs: 53.6 ± 3.22 mg GAE/mmol) compared to non-functionalized SeNPs. The significant impact of tested SeNPs on intracellular antioxidative mechanisms has been observed and it was dependent on both cell type and physico-chemical properties of SeNPs, indicating the complexity of involved mechanisms.
Ključne riječi
selenium nanoparticles; polyphenols; cytotoxicity; antioxidative activity; reactive oxygen species; reduced glutathione
Hrčak ID:
307875
URI
Datum izdavanja:
30.12.2023.
Posjeta: 151 *