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https://doi.org/10.5562/cca4016

Spectrophotometric Determination of N-acetyl-L-Cysteine in Pharmaceutical Formulations by Flow Injection and Sequential Injection Analysis: Comparison of the Methods

Maja Biocic orcid id orcid.org/0000-0002-2293-3480 ; University of Split, Faculty of Chemistry and Technology, Department of Analytical Chemistry, Ruđera Boškovića 35, 21000 Split, Croatia
Deni Karabatić ; University of Split, School of Medicine, Department of Pharmacy, Šoltanska 2, 21000 Split, Croatia
Dea Tomić ; University of Split, School of Medicine, Department of Pharmacy, Šoltanska 2, 21000 Split, Croatia
Lea Kukoc-Modun ; University of Split, Faculty of Chemistry and Technology, Department of Analytical Chemistry, Ruđera Boškovića 35, 21000 Split, Croatia


Puni tekst: engleski pdf 2.365 Kb

str. 91-97

preuzimanja: 153

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Sažetak

N-acetyl-L-cysteine (NAC), a sulfhydryl-containing compound, is mainly used as a mucolytic and as an antidote for acetaminophen overdose. Flow injection and sequential injection systems were designed and optimized with the aim of providing precise, accurate and reliable flow methods for NAC determination in pharmaceuticals with very low sample and reagent consumption. Proposed methods are based on a redox reaction wherein NAC reduces a complex of Cu(II) and bathocuproine disulfonate (BCS) to orange [Cu(BCS)2]3– complex, which absorption was measured at λmax = 483 nm. The optimized FIA and SIA configuration yielded a linear calibration curve with correlation coefficients (R2 = 0.9999 and R2 = 0.9996) in the concentration range of 3.0 × 10–7 – 3.0 × 10–5 mol L–1 and analytical frequency of 120 h–1 for the FIA method and 4.0 × 10–7 – 4.0 × 10–5 mol L–1, at sampling rate 60 h–1 for the SIA method. The proposed flow methods were successfully applied for the determination of NAC in pharmaceutical products, as the results showed good agreement with the standard method prescribed by Pharmacopoeia. Recoveries were in the range from 98.4 % to 101.9 % for the FIA method and from 97.2 % to 101.8 % for the SIA method.

Ključne riječi

N-acetyl-L-cysteine; flow injection analysis; sequential injection analysis; spectrophotometric determination; pharmaceuticals

Hrčak ID:

309282

URI

https://hrcak.srce.hr/309282

Datum izdavanja:

17.10.2023.

Posjeta: 379 *