Veterinarska stanica, Vol. 56 No. 1, 2025.
Izvorni znanstveni članak
https://doi.org/10.46419/vs.56.1.2
Antioxidant capacity and reactive oxygen metabolites in lizard (Podarcis siculus) skeletal muscle tissues - preliminary results
Ana Shek Vugrovečki
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Josip Miljković
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Ivona Žura Žaja
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Lana Pađen
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Dunja Šikić
; Division of Animal Physiology, Faculty of Science, University of Zagreb, Croatia
Tomislav Gojak
; Division of Animal Physiology, Faculty of Science, University of Zagreb, Croatia
Marko Glogoški
; Division of Animal Physiology, Faculty of Science, University of Zagreb, Croatia
Duje Lisičić
; Division of Animal Physiology, Faculty of Science, University of Zagreb, Croatia
Sofia Ana Blažević
; Division of Animal Physiology, Faculty of Science, University of Zagreb, Croatia
Jasna Aladrović
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Sažetak
There are few studies on oxidative stress in reptiles and there is a lack of basic knowledge on oxidative stress in the tissues of the widely distributed Italian wall lizard (Podarcis siculus). The aim of the present preliminary study was to test and compare the biological antioxidant potential (BAP), total superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and derivatives of reactive oxygen metabolites (d-ROM) of muscle tissue between two populations of P. siculus. Two free-living groups of eight adult males were included in each group (N=16). One group was caught on the island of Pag (at Novalja), and the second from the mainland (at Knin) in Croatia. Animals were kept in the same, optimal conditions for two months. After muscle tissue homogenisation and centrifugation in the supernatant, the activities of GSH-Px, SOD and concentrations of BAP and d-ROM were determined using commercial biochemical kits on a biochemical analyser. The results of this study showed that although there were no statistically significant differences in the values of oxidants and antioxidant parameters determined in muscle tissue supernatants between these two populations, values of all determined parameters were higher in the Pag group especially BAP values that approached statistical significance. No differences were found in d-ROM/BAP and GSH-Px/SOD ratios, but all parameters showed significant positive correlations, with the strongest positive correlation between GSH-Px and BAP, and SOD and BAP. These findings can serve as a base for future studies with a larger sample size, different tissue, different locations, between sexes or between species at the same location.
Ključne riječi
biological antioxidant potential; total superoxide dismutase; glutathione peroxidase; reactive oxygen metabolites; muscle; P. siculus
Hrčak ID:
316962
URI
Datum izdavanja:
11.6.2024.
Posjeta: 0 *