Izvorni znanstveni članak
https://doi.org/10.2478/aiht-2024-75-3836
Aqueous sage leave extract attenuates inflammation and oxidantinduced genotoxicity in human peripheral blood mononuclear cells
Ana Valenta Šobot
orcid.org/0000-0002-8429-1399
; University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
Marijana Janić
; University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
Iva Popović
; University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
Tamara Lazarević-Pašti
orcid.org/0000-0001-6220-2079
; University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
Tatjana Momić
; University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
Aleksandar Krstić
orcid.org/0000-0001-7418-6723
; University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
Jelena Filipović Tričković
orcid.org/0000-0001-5450-0842
; University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
Sažetak
Traditional medicine has used sage (Salvia officinalis L.) preparations for centuries to prevent and treat various inflammatory and oxidative stress-induced conditions. The aim of this in vitro study was to determine the bioactive properties of a sage leave extract obtained with environmentally friendly aqueous extraction and lyophilisation in primary human peripheral blood cells. To that end we measured the total phenolic and flavonoid content (TPC and TFC, respectively) with gas chromatography-mass spectrometry (GC-MS). Non-cytotoxic concentrations determined with the trypan blue assay were used to assess the antioxidant (DPPH, ABTS, and PAB assay), antigenotoxic (CBMN assay), immunomodulatory (IL-1β and TNF-α), and neuroprotective effects (AChE inhibition). The extract contained high TPC (162 mg GAE/g of dry extract) and TFC (39.47 mg QE/g of dry extract) concentrations, while β-thujone content was unexpectedly low (below 0.9 %). Strong radical-scavenging activity combined with glutathione reductase activation led to a decrease in basal and H2O2-induced oxidative stress and DNA damage. A decrease in TNF-α and increase in IL-1β levels suggest complex immunomodulatory response that could contribute to antioxidant and, together with mild AChE inhibition, neuroprotective effects. Overall, this study has demonstrated that aqueous sage leave extract reduces the levels of thujone, 1,8-cineole, pinene, and terpene ketones that could be toxic in high concentrations, while maintaining high concentrations of biologically active protective compounds which have a potential to prevent and/or treat inflammatory and oxidative stress-related conditions.
Ključne riječi
AChE inhibition; antigenotoxic effects; anti-inflammatory effects; antioxidant; Salvia officinalis L.
Hrčak ID:
318102
URI
Datum izdavanja:
19.6.2024.
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