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Genetic Polymorphisms at HLA-A, -B, and -DRB1 Loci in Han Population of Xi’an City in China

Chunmei Shen ; Key Laboratoriy of Public Health of Ministry for Forensic Sciences, Shaanxi, People's Repubilc of China
Bofeng Zhu ; Key Laboratory of Environment and Gene Related to Diseases, Shaanxi, People's Repubilc of China
Mengli Liu ; Blood Center of Shaanxi Province, Shaanxi, People's Repubilc of China
Shengbin Li ; Department of Forencis Medicine, School of Medicine, Xi'an, Shaanxi, People's Repubilc of China


Puni tekst: engleski pdf 485 Kb

verzije

str. 476-482

preuzimanja: 754

citiraj


Sažetak

Aim To determine genetic polymorphisms at human leukocyte antigen
(HLA)-A, -B, and -DRB1 loci in Han population of Xi’an city
in China.
Methods Polymerase chain reaction-based reverse line-strip sequence
specific oligonucleotide hybridization was used to determine
the alleles of HLA-A, -B, and -DRB1 in 516 unrelated, healthy individuals
of Han population in Xi’an. Allele frequencies at HLA-A,
-B, and -DRB1 loci were estimated by direct counting method. Haplotype
frequencies were calculated from genotype data by expectation
maximization.
Results A total of 14 alleles of HLA-A, 33 alleles of HLA-B, and 13
alleles of HLA-DRB1 were found. The most common alleles were
HLA-A*02 (28.39%), A*11 (19.19%), and A*24 (16.28%); HLAB*
13 (11.05%), B*15 (B62: 9.30%), and B*51 (8.53%); and HLADRB1*
15 (17.15%), DRB1*09 (13.18%), and DRB1*04 (10.85%).
The most common haplotypes of HLA-A-B-DRB1 haplotype were
HLA-A*30-B*13-DRB1*07 (3.93%), HLA-A*02-B*46-DRB1*09
(3.20%), and HLA-A*33-B*58-DRB1*17 (1.63%).
Conclusion The finding that the HLA loci are highly polymorphic
in Han population of Xi’an City may be useful for population genetics,
HLA-related studies, human identification, and paternity tests
in forensic sciences.

Ključne riječi

Han population; human leukocyte antigen; allelic frequency; haplotype frequency; Genetic polymorphisms

Hrčak ID:

29260

URI

https://hrcak.srce.hr/29260

Datum izdavanja:

15.8.2008.

Posjeta: 1.287 *