Kratko priopćenje
https://doi.org/10.2478/aiht-2024-75-3915
An in vitro evaluation of the cytotoxic potential of medicinal mushrooms against human breast cancer cell lines
Dijana Topalović
; University of Belgrade Faculty of Pharmacy, Department of Pathobiology, Belgrade, Serbia
Lada Živković
; University of Belgrade Faculty of Pharmacy, Department of Pathobiology, Belgrade, Serbia
Sunčica Borozan
; University of Belgrade Faculty of Veterinary Medicine, Department of Chemistry, Belgrade, Serbia
Juan F. Santibanez
; University of Belgrade Institute for Medical Research National Institute of the Republic of Serbia, Department of Molecular Oncology, Belgrade, Serbia 4 Bernardo O’Higgins University, Integrative Center for Biology and Applied Chemistry (CIBQA), Santiago, Chile
Biljana Spremo-Potparević
; University of Belgrade Faculty of Pharmacy, Department of Pathobiology, Belgrade, Serbia
Sažetak
Medicinal mushroom extracts, i.e. their dried biomass, have long been known as sources of bioactive compounds with positive effects on the human health. The antioxidant, antigenotoxic, antiviral, and immunomodulatory properties of the commercially available extracts Agaricus blazei auct. non Murrill (AB), Cordyceps sinensis (Berk.) Sacc. (CS), and Immune Assist (IA) have already been documented. This study, studied the influence of these three mushrooms on the viability of cell lines MCF-7, MDA-MB-231, and HS-5. The cytotoxicity of AB, CS, and IA at different concentrations (25, 50, 100, 200, 400 and 800 μg/mL) was evaluated using the MTT assay. The results showed that AB was the most effective and induced cytotoxicity in both cancer cell lines, with IC50 values of 96.7 μg/mL for MCF-7 and 368.4 μg/mL for MDA-MB-231. After treatment with CS and IA, the half-maximal inhibitory concentration was reached only in MDAMB-231 cells (IC50=613 μg/mL for CS and 343.3 μg/mL for IA). We have shown here that AB, CS and IA can suppress the growth of MCF-7 and MDA-MB-231 cell lines, while affecting the survival of healthy HS-5 cells to a much lesser extent. Our in vitro results suggested that AB, CS and IA are promising natural sources with potential anticancer activity.
Ključne riječi
cytotoxicity; Agaricus blazei; Cordyceps sinensis; Immune Assist; MTT
Hrčak ID:
324322
URI
Datum izdavanja:
19.12.2024.
Posjeta: 0 *