Izvorni znanstveni članak
https://doi.org/https://doi.org/10.5513/JCEA01/25.4.4185
Antifungal effect of the entomopathogenic fungi Beauveria bassiana on the phytopathogenic fungi Botrytis cinerea
Katarina MARTINKO
orcid.org/0000-0002-8526-8457
; University of Zagreb Faculty of Agriculture, Division of Phytomedicine, Department of Plant Pathology, Svetošimunska 25, 10000 Zagreb, Croatia
*
Anton MIHOVILOVIĆ
orcid.org/0009-0008-3577-2306
; University of Zagreb Faculty of Agriculture, Division of Phytomedicine, Department of Plant Pathology, Svetošimunska 25, 10000 Zagreb, Croatia
* Dopisni autor.
Sažetak
The use of antagonistic microorganisms in the suppression of phytopathogenic fungi represents the ultimate approach to biological control, and because of their significant beneficial effects, antagonistic microorganisms are increasingly being researched. The necrotrophic fungus Botrytis cinerea known as the causative agent of gray mold, leads to significant global
yield and post-harvest storage losses. It is a polyphagous of high risk for fungicide resistance development, which is why the use of antagonistic microorganisms a promising biocontrol strategy. Among the antagonistic microorganisms, the entomopathogenic fungus Beauveria bassiana is considered to have antagonistic properties in controlling phytopathogenic fungi due to the wide range of secondary metabolites. In order to maximize the antagonistic fungi effectiveness in field conditions, special attention was paid to researching the antagonistic mechanisms under optimal conditions for their development. The aim of this study is to test the antifungal effect of B. bassiana on pathogen B. cinerea by dual cultures method and test the production of volatile metabolites on a medium with two different pH values (pH = 5.6 and 7.2). The results of the dual culture method and testing production of volatile metabolites confirmed that the antagonistic effect of B. bassiana is significantly higher on a neutral pH medium, where the inhibition of the B. cinerea was 36%, i.e. 38% which was achieved by different antagonistic mechanisms. Microscopic analysis confirmed inhibition mechanisms (mycoparasitism and antibiosis), more significant on a neutral pH medium by B. bassiana, and B. cinerea microstructures deformations. B. bassiana isolate has a significant antagonistic potential in suppressing B. cinerea, which is the starting point for further research in vivo.
Ključne riječi
antagonism; antibiosis; Beauveria bassiana; Botrytis cinerea; mycoparasitism; inhibition
Hrčak ID:
324750
URI
Datum izdavanja:
23.12.2024.
Posjeta: 28 *