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https://doi.org/10.21857/mnlqgc3k1y
Adding Probiotics And Prebiotics To A Stable Obese Microbiota In Vitro Alters Microbial Composition
Ena Melvan
; Macquarie University, Balaclava Rd, Macquarie Park NSW 2109, Australia
*
Damir Oros
; University of Zagreb, Faculty of Food Technology and Biotechnology, Pierottijeva 6, 10000, Zagreb
Tea Vuckovic
; Research and Development Department, Metabelly, Ulica Slobode 37, 21000 Split, Croatia
Andrew P. Allen
; Macquarie University, Balaclava Rd, Macquarie Park NSW 2109, Australia
Antonio Starcevic
; University of Zagreb, Faculty of Food Technology and Biotechnology, Pierottijeva 6, 10000, Zagreb
* Dopisni autor.
Sažetak
Microbiota, defined as the ecological community of microorganisms living on and in a host, is comprised of hundreds of species that perform functions that are important to human health. The taxonomic composition of microbiota can vary dramatically through a person’s life, reflecting changes in lifestyle, nutrition, and medication usage. Changes in the microbiota have been linked to a range of diseases states including gastrointestinal and metabolic disorders, and even Alzheimer's disease. Obesity is also believed to be linked to microbiota, so there is growing interest in treating obesity by altering microbiota composition through the administration of antibiotics, probiotics and prebiotics. In this work, we assessed the effects of administration of probiotic bacteria and prebiotic fiber on the composition of gastrointestinal microbiota cultured in an in vitro system that simulates the distal part of the human colon. We hypothesized that adding probiotics and prebiotics to cultured microbiota obtained from an obese person’s feces would alter the microbial composition. We assessed changes in the microbiome using 16S rRNA gene sequencing. Results indicate that the addition of both probiotic organisms and prebiotic fiber changes the composition of gastrointestinal microbiota, as assessed by the Before-After ControlImpact design analysis. We also observed a reduction of bacterial biodiversity during the 72 hr period of the experiment in the in vitro system.
Ključne riječi
obesity; gastrointestinal microbiota; probiotic; prebiotic; bioreactor
Hrčak ID:
326023
URI
Datum izdavanja:
20.12.2024.
Posjeta: 0 *