Izvorni znanstveni članak
Immunohistochemically Detected High Expression of Matrix Metalloproteinase-2 as Predictor of Poor Prognosis in Duke’s B Colon Cancer
Željko Šundov
; Department of Internal Medicine, Split University School of Medicine, Split, Croatia
Snježana Tomić
; Department of Pathology, Split University School of Medicine, Split, Croatia
Katarina Vilović
; Department of Pathology, Split University School of Medicine, Split, Croatia
Nenad Kunac
; Department of Pathology, Split University School of Medicine, Split, Croatia
Marija Kalebić
; Department of Emergency Medicine, Split Dalmatian County, Split, Croatia
Joško Bezić
; Department of Pathology, Split University School of Medicine, Split, Croatia
Sažetak
Aim To demonstrate immunohistochemical expression of matrix metalloproteinase-
2 (MMP-2) protein in Duke’s B colon cancer and determine
its correlation with age, sex, grade, presence of vascular invasion,
and patients’ overall survival.
Method The study took place from January 1995 to December 1997. We
determined the expression of MMP-2 in 152 formalin-fixed, paraffin embedded
specimens of Duke’s B colon carcinomas by immunohistochemical
analysis using MMP-2 monoclonal antibody. Immunohistochemical
expresssion was scored semiquantitatively. Carcinomas were graded
as low or high grade. Survival time was analyzed with Kaplan-Meier
method, and the log-rank test was used to assess the differences between
groups. Cox proportional hazard regression model was used for multivariate
survival analysis.
Result Univariate analysis showed that positive staining for MMP-2,
high histological grade, vascular invasion, male sex, and age >60 years
were associated with shorter survival in patients with Duke’s B colon
cancer (P range from 0.023 to <0.001). Multivariate analysis showed that
only MMP-2 overexpression (P < 0.001; hazard ratio [HR] = 3.64) and
vascular invasion (P < 0.001; HR = 4.27) were associated with shorter
overall survival.
Conclusion Expression of MMP-2 is an important independent indicator
of shorter survival in patients with Duke’s B colon cancer and should
be taken into consideration in decision-making on the use of adjuvant
systemic therapy in patients with Duke’s B colon cancer.
Ključne riječi
MMP-2; colon cancer; immunohistochemistry; prognosis
Hrčak ID:
35100
URI
Datum izdavanja:
15.10.2008.
Posjeta: 915 *