Original scientific paper

Comparative sequence analysis of diagnostic PCR amplicons from Indian sheeppox virus

M. Parthiban ; Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, Tamil Nadu, India
R. Govindarajan ; Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, Tamil Nadu, India
S. Manoharan ; Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, Tamil Nadu, India
V. Purushothaman ; Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, Tamil Nadu, India
N. Daniel Joy Chandran ; Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, Tamil Nadu, India
A. Koteeswaran ; Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, Tamil Nadu, India

Abstract

Field samples were identified by agar gel precipitation and polymerase chain reaction (PCR) as positive for sheeppox virus. Envelope protein gene PCR amplicons (192 bp) of Indian sheeppox viruses isolated in 1997 and 2003 were sequenced to analyse nucleotide divergence. Analysis revealed that 2003 isolates possessed 100% nucleotide identity to each other, but only 95% nucleotide and amino acid were identity to an isolate from 1997. These Indian isolates were unique from other capripox virus sequences in that they contained a single codon insertion. Consistent with previous findings, the results indicate that recent sheepox virus isolates in India are more similar to previously described sheepox virus than to goatpox viruses from India and elsewhere.

Keywords

sheeppox virus; India; nucleotide sequence; envelope protein; phylogenetic analysis

Hrčak ID:

37432

URI

https://hrcak.srce.hr/37432

Publication date:

20.6.2005.

Article data in other languages: croatian

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