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Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source

X. Zou ; State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P. R. China; College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, P. R. China
C.-F. Chen ; State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P. R. China
H.-F. Hang ; State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P. R. China
J. Chu ; State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P. R. China
Y.-P. Zhuang ; State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P. R. China
S.-L. Zhang ; State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P. R. China

Sažetak

A novel source of biological nitrogen produced by multi-strain fermentation was developed for erythromycin production.The nitrogen composition of corn steep liquor, biological nitrogen and soybean flour was optimized by using Response surface methodology (RSM) firstly. A Box-Behnken design was used to estimate the optimum nitrogen composition for the production of erythromycin as follows: corn steep liquor: γ =5.1 g L-1, biological nitrogen γ = 5.96 g L-1 and soybean flour γ = 24.17 g L-1.
Based on the optimum nitrogen source, the constant glucose fed-batch and pH control fed-batch strategies were used to further optimize the erythromycin production in 50 L stirred bioreactor, respectively.It was found that pH control could serve as an effective
control strategy for erythromycin production, and the maximum erythromycin production was 8528 U mL-1 at 190 h.This work demonstrates that the new medium formulation based on a cheap nitrogen source, biological nitrogen, is a potential alternative for
economic erythromycin production on a large scale.

Ključne riječi

Biological nitrogen; erythromycin production; fed-batch strategy; medium optimization; response surface methodology; Saccharopolyspora erythraea

Hrčak ID:

49490

URI

https://hrcak.srce.hr/49490

Datum izdavanja:

25.3.2010.

Posjeta: 2.105 *