Izvorni znanstveni članak
Forensic Efficiency Parameters for the 15 STR Loci in the Population of the Island of Cres (Croatia)
Natalija Novokmet
; Institute for Anthropological Research, Zagreb, Croatia
Damir Marjanović
; Genos, DNA Laboratory, Zagreb, Croatia
Vedrana Škaro
; Genos, DNA Laboratory, Zagreb, Croatia
Petar Projić
; Genos, DNA Laboratory, Zagreb, Croatia
Gordan Lauc
; DNA Laboratory, School of Medicine, »J. J. Strossmayer« University of Osijek, Osijek, Croatia
Blaženka Grahovac
; Department of Pathology and Pathological Anatomy, School of Medicine, University of Rijeka, Rijeka Croatia
Bojana Mohar
; Department of Pathology and Pathological Anatomy, School of Medicine, University of Rijeka, Rijeka Croatia
Miljenko Kapović
; Department of Biology and Medical Genetics, School of Medicine, University of Rijeka, Rijeka Croatia
Pavao Rudan
; Institute for Anthropological Research, Zagreb, Croatia
Sažetak
Forensic parameters based on 15 AmpFlSTR Identifiler short tandem repeat (STR) loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818 and FGA) were evaluated in the sample of 122 unrelated, autochthonous, adult individuals from the Island of Cres (Croatia). PCR amplification was performed with the AmpFlSTR Identifiler PCR Amplification Kit and the amplified products were separated and detected using the ABI 3130 DNA genetic analyzer. The agreement with Hardy Weinberg Equilibrium (HWE) was confirmed for all loci (p>0.05). The combined power of discrimination (PD) and the combined power of exclusion (PE) for the 15 tested STR loci were 0.99999999999999997988728679 and 0.999997397, respectively. According to the presented data, D18S51 proved to be the most informative marker followed by markers D2S1338 and D21S11. Interpopulation comparisons in allele frequencies with other East Adriatic Islands revealed significant differences for all analyzed population pairs ranging from 4 loci (Cres vs. Hvar) to 1 locus (Cres vs. Krk). Furthermore, allele frequencies comparisons of Cres and Croatian mainland revealed the lack of statistically significant differences at all studied loci. The results of the current study indicate that the examined fifteen STR loci are useful genetic markers for individual identification and paternity testing in Croatian population from the Island of Cres.
Ključne riječi
STRS; AmpFlSTR identifiler; forensic parameters; the Island of Cres; Croatia
Hrčak ID:
51520
URI
Datum izdavanja:
1.12.2009.
Posjeta: 1.453 *