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Laser Induced Fluorescence of Carious Lesion Porphyrins
Danijela Matošević
orcid.org/0000-0003-3104-5749
; Stomatološki fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Zrinka Tarle
; Stomatološki fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Snežana Miljanić
; Zavod za analitičku kemiju Prirodoslovno-matematičkog fakulteta Sveučilišta u Zagrebu Horvatovac 102a, Zagreb, Hrvatska
Zlatko Meić
; Zavod za analitičku kemiju Prirodoslovno-matematičkog fakulteta Sveučilišta u Zagrebu Horvatovac 102a, Zagreb, Hrvatska
Lana Pichler
; Laboratorij za femtosekundnu lasersku spektroskopiju Instituta za fiziku, Bijenička cesta 46, Zagreb, Hrvatska
Goran Pichler
; Laboratorij za femtosekundnu lasersku spektroskopiju Instituta za fiziku, Bijenička cesta 46, Zagreb, Hrvatska
Sažetak
Objectives: This paper reports the preliminary results of the measurements of laser induced fluorescence in several porphyrin solutions. Coproporphyrin and uroporphyrin are common constituents
of carious lesions. Their property to exhibit fluorescence when irradiated with a light of certain wavelength could be used as a means to detect carious lesions. Materials and methods: Absorption coefficient measurements of coproporphyrin I dihydrochloride and uroporphyrin I dihydrochloride solutions were performed under different pH conditions in order to identify spectral regions for effective laser excitation. Lasers with discrete wavelengths at 420 nm, 473 nm and 532 nm were used for the induction of the fluorescence. Results: At all laser wavelengths interesting fluorescence bands peaking at 591 nm, 619 nm and 652 nm for coproporphyrin and at 617 nm and 680 nm for uroporphyrin were observed. Conclusions: When combined together all bands should correspond to the spectral band structures found in real carious lesions.
Ključne riječi
Porphyrins; Coproporphyrins; Uroporphyrins; Dental Caries; Spectrometry, Fluorescence
Hrčak ID:
55633
URI
Datum izdavanja:
18.6.2010.
Posjeta: 2.790 *