Original scientific paper

Cell surface changes associated with in vitro capacitation and acrosome reaction of goat epididymal sperm by a marine bio-active compound from the snail Telescopium telescopium

Samata Maji ; Department of Veterinary Gynaecology and Obstetrics, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kshudiram Bose Sarani, Belgachia, Kolkata, West Bengal, India
Uttam Datta ; Department of Veterinary Gynaecology and Obstetrics, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kshudiram Bose Sarani, Belgachia, Kolkata, West Bengal, India
Manik L. Hembram ; Department of Veterinary Gynaecology and Obstetrics, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kshudiram Bose Sarani, Belgachia, Kolkata, West Bengal, India

Abstract

A protein factor, spermatheca fraction fi fty, isolated from the spermatheca/ovotestis gland of a marine mollusc Telescopium telescopium by 50% ammonium sulphate precipitation was capable of inducing a capacitation and acrosome reaction of washed goat cauda epididymal spermatozoa in vitro. The effect of this isolated bio-active compound on goat sperm cells was comparable to heparin and concanavalinA. The number of acrosome reacted spermatozoa treated with heparin, concanavalinA and spermatheca fraction fifty increased steadily and signifi cantly (P<0.001) over the extended period of incubation. Observation concluded that spermatheca fraction fifty could be used for the evaluation of sperm plasmamembrane integrity and/or acrosomal integrity in vitro.

Keywords

Telescopium telescopium; sperm; capacitation; acrosome reaction

Hrčak ID:

62143

URI

https://hrcak.srce.hr/62143

Publication date:

26.10.2010.

Article data in other languages: croatian

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